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Accepted (AM)Open Access (License Unspecified), Open
Journal article
Autoantibodies to Recombinant Human CTL2 in Autoimmune Hearing Loss
The Laryngoscope, v 119(5), pp 924-932
01 May 2009
PMID: 19319905
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Objectives/Hypothesis: Choline transporter-like protein 2 (CTL2), a 68-72 kDa inner-ear membrane glycoprotein, is a candidate target antigen in autoimmune hearing loss (AIHL). The objective of this study was to test recombinant human CTL2 as a potential target for the detection of human autoantibodies in patients with AIHL.
Study Design: In vitro assay development.
Methods: Human inner ear CTL2 mRNA was cloned into baculovirus and used to infect insect cells. Immunofluorescence and western blotting were used to determine optimal expression of recombinant human CTL2 (rHuCTL2) in insect cells. AIHL patient sera of known reactivity with guinea pig inner ear were tested for antibodies to purified rHuCTL2 on western blots. Sera from normal hearing donors were used as controls.
Results: The rHuCTL2 protein migrated as three bands: a core protein of 62kDa and two N-glycosylated bands at 66 and 70 kDa. Sera from 6/12 (50%) of AIHL patients with antibody to the 68-72 kDa inner-ear protein or to supporting cells also have antibody to rHuCTL2. Four of the four patients with antibody to rHuCTL2 responded to corticosteroids, whereas 4/8 that lacked antibody to rHuCTL2 did not. Among normal human sera, 80% were negative; binding was barely detectable in 3/15 (20%).
Conclusions: The rHuCTL2 protein can be produced efficiently and used as a substrate for testing human sera. Antibodies to rHuCTL2 were detected in 50% of inner-ear-reactive AIHL sera. Additionally, circulating antibody to rHuCTL2 is with associated response to corticosteroids in some AIHL patients.
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Details
- Title
- Autoantibodies to Recombinant Human CTL2 in Autoimmune Hearing Loss
- Creators
- Pavan K. Kommareddi - University of Michigan–Ann ArborThankam S. Nair - University of Michigan–Ann ArborMounica Vallurupalli - Kresge Hearing Research InstituteSteven A. Telian - University of Michigan–Ann ArborH. Alexander Arts - University of Michigan–Ann ArborHussam K. El-Kashlan - University of Michigan–Ann ArborRobert T. Sataloff - Drexel UniversityThomas E. Carey - Kresge Hearing Research Institute
- Publication Details
- The Laryngoscope, v 119(5), pp 924-932
- Publisher
- Wiley
- Number of pages
- 9
- Grant note
- Autoimmune Sensorineural Hearing Loss Research Fund 1101 DC03686 / NIH NIDCD; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute on Deafness & Other Communication Disorders (NIDCD) T32DC000011 / NATIONAL INSTITUTE ON DEAFNESS AND OTHER COMMUNICATION DISORDERS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute on Deafness & Other Communication Disorders (NIDCD) 1P30 AR048310 / NIH Rheumatic Core Diseases Center; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA P30AR048310 / NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Arthritis & Musculoskeletal & Skin Diseases (NIAMS) Ruth and Lynn Townsend Family Fund P30 DC05188; T32 DC00011 / NIDCD Research Center Core; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute on Deafness & Other Communication Disorders (NIDCD)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- College of Medicine
- Web of Science ID
- WOS:000265866000015
- Scopus ID
- 2-s2.0-66349115338
- Other Identifier
- 991019312455804721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Medicine, Research & Experimental
- Otorhinolaryngology