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Journal article
Automated Measurement of Cobblestone Morphology for Characterizing Stem Cell Derived Retinal Pigment Epithelial Cell Cultures
Journal of ocular pharmacology and therapeutics, v 32(5), pp 331-339
01 Jun 2016
PMID: 27191513
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Purpose:
Assessing the morphologic properties of cells in microscopy images is an important task to evaluate cell health, identity, and purity. Typically, subjective visual assessments are accomplished by an experienced researcher. This subjective human step makes transfer of the evaluation process from the laboratory to the cell manufacturing facility difficult and time consuming.
Methods:
Automated image analysis can provide rapid, objective measurements of cultured cells, greatly aiding manufacturing, regulatory, and research goals. Automated algorithms for classifying images based on appearance characteristics typically either extract features from the image and use those features for classification or use the images directly as input to the classification algorithm. In this study we have developed both feature and nonfeature extraction methods for automatically measuring “cobblestone” structure in human retinal pigment epithelial (RPE) cell cultures.
Results:
A new approach using image compression combined with a Kolmogorov complexity-based distance metric enables robust classification of microscopy images of RPE cell cultures. The automated measurements corroborate determinations made by experienced cell biologists. We have also developed an approach for using steerable wavelet filters for extracting features to characterize the individual cellular junctions.
Conclusions:
Two image analysis techniques enable robust and accurate characterization of the cobblestone morphology that is indicative of viable RPE cultures for therapeutic applications.
Metrics
Details
- Title
- Automated Measurement of Cobblestone Morphology for Characterizing Stem Cell Derived Retinal Pigment Epithelial Cell Cultures
- Creators
- Rohini Joshi - Drexel UniversityWalter Mankowski - Drexel UniversityMark Winter - Drexel UniversityJanmeet S. Saini - Neural Stem Cell InstituteTimothy A. Blenkinsop - Mount Sinai HospitalJeffrey H. Stern - Neural Stem Cell InstituteSally Temple - Neural Stem Cell InstituteAndrew R. Cohen - Drexel University
- Publication Details
- Journal of ocular pharmacology and therapeutics, v 32(5), pp 331-339
- Publisher
- Mary Ann Liebert, Inc
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Electrical and Computer Engineering
- Web of Science ID
- WOS:000377399500012
- Scopus ID
- 2-s2.0-84974653205
- Other Identifier
- 991019169170004721
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InCites Highlights
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Ophthalmology
- Pharmacology & Pharmacy