Files and links (1)
Published, Version of Record (VoR)CC BY-NC-SA V4.0, Open
Journal article
Axonal Localization of Transgene mRNA in Mature PNS and CNS Neurons
The Journal of neuroscience, v 31(41), pp 14481-14487
12 Oct 2011
PMID: 21994364
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Axonal mRNA transport is robust in cultured neurons but there has been limited evidence for this in vivo. We have used a genetic approach to test for in vivo axonal transport of reporter mRNAs. We show that beta-actin's 3'-UTR can drive axonal localization of GFP mRNA in mature DRG neurons, but mice with gamma-actin's 3'-UTR show no axonal GFP mRNA. Peripheral axotomy triggers transport of the beta-actin 3'-UTR containing transgene mRNA into axons. This GFP-3'-beta-actin mRNA accumulates in injured PNS axons before activation of the transgene promoter peaks in the DRG. Spinal cord injury also increases axonal GFP signals in mice carrying this transgene without any increase in transgene expression in the DRGs. These data show for the first time that the beta-actin 3'-UTR is sufficient for axonal localization in both PNS and CNS neurons in vivo.
Metrics
Details
- Title
- Axonal Localization of Transgene mRNA in Mature PNS and CNS Neurons
- Creators
- Dianna E. Willis - Burke Medical Research InstituteMei Xu - Emory UniversityChristopher J. Donnelly - University of DelawareChhavy Tep - The Ohio State UniversityMarvin Kendall - Community Health Systems - Dupont HospitalMarina Erenstheyn - Community Health Systems - Dupont HospitalArthur W. English - AID AtlantaN. Carolyn Schanen - University of DelawareCatherine B. Kirn-Safran - University of DelawareSung Ok Yoon - The Ohio State UniversityGary J. Bassell - Emory UniversityJeffery L. Twiss - Drexel University
- Publication Details
- The Journal of neuroscience, v 31(41), pp 14481-14487
- Publisher
- Soc Neuroscience
- Number of pages
- 7
- Grant note
- R21-NS045880; R01-NS041596; K99-NR010797; R21-NS060098; P20-RR15588 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA R01NS041596 / NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS) K99NR010797 / NATIONAL INSTITUTE OF NURSING RESEARCH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Nursing Research (NINR) P20RR020173 / NATIONAL CENTER FOR RESEARCH RESOURCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Center for Research Resources (NCRR) TB2-0602 / Christopher and Dana Reeve Foundation
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000296019600005
- Scopus ID
- 2-s2.0-80054012330
- Other Identifier
- 991021892106104721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Neurosciences