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Journal article
Axonal amphoterin mRNA is regulated by translational control and enhances axon outgrowth
The Journal of neuroscience, v 35(14), pp 5693-5706
08 Apr 2015
PMID: 25855182
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
High mobility group (HMG) proteins concentrate in the nucleus, interacting with chromatin. Amphoterin is an HMG protein (HMGB1) that has been shown to have extranuclear functions and can be secreted from some cell types. Exogenous amphoterin can increase neurite growth, suggesting that the secreted protein may have growth promoting activities in neurons. Consistent with this, we show that depletion of amphoterin mRNA from cultured adult rat DRG neurons attenuates neurite outgrowth, pointing to autocrine or paracrine mechanisms for its growth-promoting effects. The mRNA encoding amphoterin localizes to axonal processes and we showed recently that its 3'-UTR is sufficient for axonal localization of heterologous transcripts (Donnelly et al., 2013). Here, we show that amphoterin mRNA is transported constitutively into axons of adult DRG neurons. A preconditioning nerve injury increases the levels of amphoterin protein in axons without a corresponding increase in amphoterin mRNA in the axons. A 60 nucleotide region of the amphoterin mRNA 3'-UTR is necessary and sufficient for its localization into axons of cultured sensory neurons. Amphoterin mRNA 3'-UTR is also sufficient for axonal localization in distal axons of DRG neurons in vivo. Overexpression of axonally targeted amphoterin mRNA increases axon outgrowth in cultured sensory neurons, but axon growth is not affected when the overexpressed mRNA is restricted to the cell body.
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Details
- Title
- Axonal amphoterin mRNA is regulated by translational control and enhances axon outgrowth
- Creators
- Tanuja T Merianda - Department of Anatomy and Neurobiology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19129Jennifer Coleman - Alfred I. duPont Hospital for ChildrenHak Hee Kim - Alfred I. duPont Hospital for ChildrenPabitra Kumar Sahoo - University of South CarolinaCynthia Gomes - Department of Biological Sciences, University of South Carolina, Columbia, South Carolina 29208Paul Brito-Vargas - University of South CarolinaHeikki Rauvala - University of HelsinkiArmin Blesch - University of California San DiegoSoonmoon Yoo - Nemours Biomedical Research, Alfred I. duPont Hospital for Children, Wilmington, Delaware 19803Jeffery L Twiss - University of South Carolina
- Publication Details
- The Journal of neuroscience, v 35(14), pp 5693-5706
- Publisher
- Society for Neuroscience
- Grant note
- R25 GM066526 / NIGMS NIH HHS R25-GM066526 / NIGMS NIH HHS R01 NS041596 / NINDS NIH HHS P20-GM103446 / NIGMS NIH HHS R21 NS085691 / NINDS NIH HHS R01-NS041596 / NINDS NIH HHS R21-NS085691 / NINDS NIH HHS P20 GM103446 / NIGMS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000353054900021
- Scopus ID
- 2-s2.0-84929353452
- Other Identifier
- 991021892106304721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Neurosciences