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Journal article
Axonal localization of the fragile X family of RNA binding proteins is conserved across mammals
Journal of comparative neurology (1911), v 528(3), pp 502-519
15 Feb 2020
PMID: 31502255
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Spatial segregation of proteins to neuronal axons arises in part from local translation of mRNAs that are first transported into axons in ribonucleoprotein particles (RNPs), complexes containing mRNAs and RNA binding proteins. Understanding the importance of local translation for a particular circuit requires not only identifying axonal RNPs and their mRNA cargoes, but also whether these RNPs are broadly conserved or restricted to only a few species. Fragile X granules (FXGs) are axonal RNPs containing the fragile X related family of RNA binding proteins along with ribosomes and specific mRNAs. FXGs were previously identified in mouse, rat, and human brains in a conserved subset of neuronal circuits but with species-dependent developmental profiles. Here, we asked whether FXGs are a broadly conserved feature of the mammalian brain and sought to better understand the species-dependent developmental expression pattern. We found FXGs in a conserved subset of neurons and circuits in the brains of every examined species that together include mammalian taxa separated by up to 160 million years of divergent evolution. A developmental analysis of rodents revealed that FXG expression in frontal cortex and olfactory bulb followed consistent patterns in all species examined. In contrast, FXGs in hippocampal mossy fibers increased in abundance across development for most species but decreased across development in guinea pigs and members of theMusgenus, animals that navigate particularly small home ranges in the wild. The widespread conservation of FXGs suggests that axonal translation is an ancient, conserved mechanism for regulating the proteome of mammalian axons.
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Details
- Title
- Axonal localization of the fragile X family of RNA binding proteins is conserved across mammals
- Creators
- Katherine A. Shepard - Drexel UniversityLulu I. T. Korsak - Drexel UniversityDanielle DeBartolo - Drexel UniversityMichael R. Akins - Drexel University
- Publication Details
- Journal of comparative neurology (1911), v 528(3), pp 502-519
- Publisher
- Wiley
- Number of pages
- 18
- Grant note
- R01 EY022987 / NEI NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Eye Institute (NEI) R00 MH090237 / NIMH NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Mental Health (NIMH)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology
- Web of Science ID
- WOS:000578791900010
- Scopus ID
- 2-s2.0-85074032512
- Other Identifier
- 991019168995304721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Neurosciences
- Zoology