Journal article
Axonal transport of neural membrane protein 35 mRNA increases axon growth
Journal of cell science, v 126(1), pp 90-102
01 Jan 2013
PMID: 23097042
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Many neuronal mRNAs are transported from cell bodies into axons and dendrites. Localized translation of the mRNAs brings autonomy to these processes that can be vast distances from the cell body. For axons, these translational responses have been linked to growth and injury signaling, but there has been little information about local function of individual axonally synthesized proteins. In the present study, we show that axonal injury increases levels of the mRNA encoding neural membrane protein 35 (NMP35) in axons, with a commensurate decrease in the cell body levels of NMP35 mRNA. The 3' untranslated region (3'UTR) of NMP35 is responsible for this localization into axons. Previous studies have shown that NMP35 protein supports cell survival by inhibiting Fas-ligand-mediated apoptosis; however, these investigations did not distinguish functions of the locally generated NMP35 protein. Using axonally targeted versus cell-body-restricted NMP35 constructs, we show that NMP35 supports axonal growth, and overexpression of an axonally targeted NMP35 mRNA is sufficient to increase axonal outgrowth.
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Details
- Title
- Axonal transport of neural membrane protein 35 mRNA increases axon growth
- Creators
- Tanuja T. Merianda - Drexel Univ, Dept Biol, Philadelphia, PA 19104 USADeepika Vuppalanchi - University of DelawareSoonmoon Yoo - Alfred I du Pont Hosp Children, Wilmington, DE 19803 USAArmin Blesch - University of California, San DiegoJeffery L. Twiss - University of South Carolina
- Publication Details
- Journal of cell science, v 126(1), pp 90-102
- Publisher
- COMPANY OF BIOLOGISTS LTD
- Number of pages
- 13
- Grant note
- R01NS041596 / NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS) P116 / International Foundation for Research in Paraplegia Miriam and Sheldon G. Adelson Medical Research Foundation 2520 / Paralyzed Veterans of America Spinal Cord Research Foundation R01-NS041596 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000316460800011
- Scopus ID
- 2-s2.0-84875589158
- Other Identifier
- 991021892105904721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Cell Biology