Logo image
Back
BDNF and NT4/5 promote survival and neurite outgrowth of pontocerebellar mossy fiber neurons
Journal article   Open access

BDNF and NT4/5 promote survival and neurite outgrowth of pontocerebellar mossy fiber neurons

Sylvia A Rabacchi, Barbara Kruk, Jason Hamilton, Catrina Carney, John R Hoffman, Sheryl L Meyer, Joe E Springer and Douglas H Baird
Journal of neurobiology, v 40(2), pp 254-269
Aug 1999
DOI: https://doi.org/10.1002/(SICI)1097-4695(199908)40:2<254::AID-NEU11>3.0.CO;2-4
PMID: 10413455
Featured in Collection :   UN Sustainable Development Goals @ Drexel
url
https://doi.org/10.1002/(sici)1097-4695(199908)40:2<254::aid-neu11>3.0.co;2-4View
Published, Version of Record (VoR)Maybe Open Access (Publisher Bronze),  Open
url
https://doi.org/10.1002/(SICI)1097-4695(199908)40:2<254::AID-NEU11>3.0.CO;2-4View
Published, Version of Record (VoR) Open

Abstract

mossy fibers development growth cones neurotrophins cerebellum basilar pontine nuclei
The neurotrophins nerve growth factor (NGF), brain‐derived neurotrophic factor (BDNF), neurotrophin‐3 (NT3), and NT4/5 are all found in the developing cerebellum. Granule cells, the major target neurons of mossy fibers, express BDNF during mossy fiber synaptogenesis. To determine whether neurotrophins contribute to the development of cerebellar afferent axons, we characterized the effects of neurotrophins on the growth of mossy fiber neurons from mice and rats in vitro. For a mossy fiber source, we used the basilar pontine nuclei (BPN), the major source of cerebellar mossy fibers in mammals. BDNF and NT4/5 increased BPN neuron survival, neurite outgrowth, growth cone size, and elongation rate, while neither NT3 nor NGF increased survival or outgrowth. In addition, BDNF and NT4/5 reduced the size of neurite bundles. Consistent with these effects, in situ hybridization on cultured basilar pontine neurons revealed the presence of mRNA encoding the TrkB receptor which binds both BDNF and NT4/5 with high affinity. We detected little or no message encoding the TrkC receptor which preferentially binds NT3. BDNF and NT4/5 also increased TrkB mRNA levels in BPN neurons. In addition to previously established functions as an autocrine/paracrine trophic factor for granule cells, the present results indicate that cerebellar BDNF may also act as a target‐derived trophic factor for basilar pontine mossy fibers. © 1999 John Wiley & Sons, Inc. J Neurobiol 40: 254–269, 1999

Metrics

12 Record Views
39 citations in Web of Science
8 citations in Scopus

Details

UN Sustainable Development Goals (SDGs)

This publication has contributed to the advancement of the following goals:

#3 Good Health and Well-Being

InCites Highlights

Data related to this publication, from InCites Benchmarking & Analytics tool:

Collaboration types
Industry collaboration
Domestic collaboration
Web of Science research areas
Neurosciences
Logo image