Journal article
Bacterial Attachment and Viscoelasticity: Physicochemical and Motility Effects Analyzed Using Quartz Crystal Microbalance with Dissipation (QCM-D)
Environmental science & technology, v 47(1), pp 398-404
02 Jan 2013
PMID: 23186151
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
This investigation is focused on the combined effect of bacterial physicochemical characteristics and motility on cell adhesion and deposition using a flow-through quartz crystal microbalance with dissipation (QCM-D). Three model flagellated strains with different degrees of motility were selected, including a highly motile Escherichia coli K12 MG1655, an environmental strain Sphingomonas wittichii RW1, and a nonmotile (with paralyzed flagella) Escherichia coli K12 MG1655 Delta motA that is incapable of encoding the motor torque generator for flagellar movement. Of the three strains, S. wittichii RW1 is highly hydrophobic, while E. coli strains are equally hydrophilic. Consideration of the hydrophobicity provides an alternative explanation for the bacterial adhesion behavior. QCM-D results show that motility is a critical factor in determining bacterial adhesion, as long as the aquatic chemical conditions are conducive for motility and the substratum and bacterial surface are similarly hydrophobic or hydrophilic. Once their properties are not similar, the contribution of hydrophobic interactions becomes more pronounced. QCM-D results suggest that during adhesion of the hydrophobic bacterium, S. wittichii RW1, the initial step of adhesion and maturation of bacteria substratum interaction on hydrophilic surface includes a dynamic change of the viscoelastic properties of the bond bacterium-surface becoming more viscously oriented.
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Details
- Title
- Bacterial Attachment and Viscoelasticity: Physicochemical and Motility Effects Analyzed Using Quartz Crystal Microbalance with Dissipation (QCM-D)
- Creators
- Jenia Gutman - Ben-Gurion University of the NegevSharon L. Walker - University of California, RiversideViatcheslav Freger - Technion – Israel Institute of TechnologyMoshe Herzberg - Ben-Gurion University of the Negev
- Publication Details
- Environmental science & technology, v 47(1), pp 398-404
- Publisher
- American Chemical Society; Washington, DC
- Number of pages
- 7
- Grant note
- Israeli Water Authority U.S. Fulbright Program 1360/10 / Israel Science Foundation
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- College of Engineering
- Web of Science ID
- WOS:000313220300051
- Scopus ID
- 2-s2.0-84871755141
- Other Identifier
- 991021229903804721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Engineering, Environmental
- Environmental Sciences