Journal article
Bacterial Identification and Visualization of Bacterial Biofilms Adjacent to Fracture Sites After Internal Fixation
Genetic testing and molecular biomarkers, v 26(2)
Feb 2022
PMID: 35225678
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The primary aims of this study were to determine if any correlation exists in cases of fracture fixation among: (1) bacterial profiles recovered from the instrumentation and adjacent tissues; (2) the type of orthopedic injury; and (3) the clinical outcome-union versus nonunion. A secondary goal was to compare culture and molecular diagnostics for identifying the bacterial species present following fracture fixation.
Single-institution, prospective case-control cohort study.
Single level 1 trauma center.
Forty-nine bony nonunion cases undergoing revision internal fixation and 45 healed fracture controls undergoing removal of hardware.
Bacterial infection was detected by standard microbial culture methods and by a pan-eubacterial domain, molecular diagnostic (MDx) assay. Confirmation of culture and MDx results was achieved with bacterial ribosomal 16S rRNA fluorescence
hybridization (FISH) to visualize bacterial biofilms.
MDx and microbial culture methods results were the primary study outcomes.
Ninety-four percent of the nonunion cohort and 93% of the union cohort had bacteria detected by the MDx. Seventy-eight percent of the nonunion cases and 69% of the controls were culture negative, but MDx positive. Although no significant differences in bacterial composition were observed between the cases and controls, differences were observed when cases were divided by comorbidities.
The MDx is more sensitive than microbial culture in detecting bacterial presence. The lack of significantly different findings with regard to bacterial profile identified between the cases and controls suggests that host factors and environmental conditions are largely responsible for determining if bony union will occur.
Diagnostic Level III. See Instructions for Authors for a complete description of levels of evidence.
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Details
- Title
- Bacterial Identification and Visualization of Bacterial Biofilms Adjacent to Fracture Sites After Internal Fixation
- Creators
- Michael P Palmer - Christ HospitalDaniel T Altman - Allegheny General HospitalGregory T Altman - Allegheny General HospitalJeffrey J Sewecke - Allegheny General HospitalCourtney Saltarski - Southwestern Medical CenterLaura Nistico - Allegheny General HospitalRachael Melton-Kreft - Allegheny General HospitalFen Z Hu - Center for Biofilms and Surgical Infections, Center for Genomic Sciences, and Center for Advanced Microbial Processing, Institute of Molecular Medicine and Infectious Disease, Philadelphia, Pennsylvania, USA.Garth D Ehrlich - Genomic (Brazil)
- Publication Details
- Genetic testing and molecular biomarkers, v 26(2)
- Publisher
- Mary Ann Liebert
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology; Orthopedic/Orthopaedic Surgery
- Web of Science ID
- WOS:000767518100003
- Scopus ID
- 2-s2.0-85125428605
- Other Identifier
- 991019168450504721
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InCites Highlights
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Genetics & Heredity