Journal article
Beta interferon plus gamma interferon efficiently reduces acyclovir-resistant herpes simplex virus infection in mice in a T-cell-independent manner
Journal of general virology, v 91(3), pp 591-598
01 Mar 2010
PMID: 19906941
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Acyclovir (ACV)-resistant herpes simplex virus type 1 (HSV-1) causes severe diseases in immunocompromised patients, so identification of new therapies is needed. Interferons (IFNs) are used to treat several other viral infections in the clinic, and IFN-beta and IFNI-gamma are known to cooperatively reduce wild-type HSV-1 replication in the corneas of immunocompetent mice. Because IFN-gamma has been shown to exert an antiviral effect mostly through T cells, whether combined IFN treatment can still inhibit ACV-resistant HSV-1 replication, especially in immunocompromised hosts, is unknown. The present study evaluated the efficacy of combined FIN treatment on ACV-resistant HSV-1 mutants. In vitro results showed that IFN-beta acted synergistically with IFN-gamma to inhibit HSV-1 replication in both human and mouse cell lines. Some ACV-resistant mutants were actually hypersensitive to combined IFN treatment. In vivo results showed that topical treatment with a low dose of IFN-beta plus IFN-gamma (200 U each) on mouse corneas efficiently reduced the viral loads by up to 4, 4 and 3 logs, respectively, in the eyes, trigeminal ganglia and brainstems of wild-type and also immunocompromised nude mice infected or co-infected with ACV-resistant HSV-1 in a manner independent of T cells. A highly efficient reduction in HSV acute replication by combined IFN treatment led to a dramatic decrease in subsequent virus reactivation from neural tissues, trigeminal ganglia, brainstems and spinal cords of latently infected mice. Thus, a combination of IFN-beta and IFN-gamma could be a potential treatment for ACV-resistant HSV-1 in immunocompromised patients.
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Details
- Title
- Beta interferon plus gamma interferon efficiently reduces acyclovir-resistant herpes simplex virus infection in mice in a T-cell-independent manner
- Creators
- Wen-Yen Huang - National Cheng Kung UniversityYing-Hsiu Su - Drexel UniversityHui-Wen Yao - National Cheng Kung UniversityPing Ling - National Cheng Kung UniversityYuk-Ying Tung - National Cheng Kung UniversityShih-Heng Chen - National Cheng Kung UniversityXiaohe Wan - Drexel Univ, Coll Med, Dept Microbiol & Immunol, Doylestown, PA 18901 USAShun-Hua Chen - National Cheng Kung University
- Publication Details
- Journal of general virology, v 91(3), pp 591-598
- Publisher
- SOC GENERAL MICROBIOLOGY
- Number of pages
- 8
- Grant note
- NSC-97-2320-B-006-010-MY3 / National Science Council in Taiwan; Ministry of Science and Technology, Taiwan
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000275587200001
- Scopus ID
- 2-s2.0-76849089520
- Other Identifier
- 991019167809404721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biotechnology & Applied Microbiology
- Virology