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Bidirectional inhibitory interactions between the embryonic chicken metanephros and lumbosacral nerves in vitro
Journal article   Open access   Peer reviewed

Bidirectional inhibitory interactions between the embryonic chicken metanephros and lumbosacral nerves in vitro

Lee Silver, Liang Qiang, Robert Loudon and Gianluca Gallo
Developmental dynamics, v 231(1), pp 190-198
01 Sep 2004
PMID: 15305299
url
https://doi.org/10.1002/dvdy.20111View
Published, Version of Record (VoR)Maybe Open Access (Publisher Bronze) Open

Abstract

During chicken embryonic development the metanephros forms from the uretic duct at embryonic day (E) 7. As the metanephric tissue develops between E7 and E10, it comes into close apposition with lumbosacral nerves. Coculturing of metanephric and nerve explants demonstrated that the Schwann cells of the sciatic nerve inhibit the migration of metanephric cells in a contact-dependent manner. Conversely, metanephric cells inhibit dorsal root ganglion axon extension in a contact-dependent manner. However, metanephric cells are not inhibited by contact with growth cones or axons. Dorsal root ganglion growth cones become sensitive to the inhibitory signals on the surfaces of metanephric cells around E8, a time when the metanephros is expanding into the territory occupied by nerves in vivo. These observations demonstrate inhibitory bidirectional tissue-tissue interactions in vitro and provide a novel model system for the study of contact-based guidance of both neuronal and non-neuronal cell migration.

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Web of Science research areas
Anatomy & Morphology
Developmental Biology
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