Journal article
Biochemical evaluation of wound healing efficacy of cold plasma-conditioned media under different operational conditions
Journal of physics. D, Applied physics
27 Jun 2024
Abstract
Abstract Wound healing is a dynamic and intricate biological process crucial for tissue repair and regeneration. This study explores the potential therapeutic impact of non-thermal plasma generated by a hand-held cold atmospheric pressure plasma jet (C-APPJ) source on fibroblast cells (NIH/3T3) in vitro. The sequential phases of wound healing — inflammation, cell proliferation, and tissue remodelling, were assessed in the context of cell migration and oxidative stress dynamics. Typically, plasma generates a mixture of several reactive oxygen/nitrogen (ROS/RNS) species. If the redox balance tilts towards higher levels of pro-oxidants than the counteracting anti-oxidants, cells face oxidative stress. The present study investigates the safety and efficacy of C-APPJ under distinct operating conditions (Argon (GI) and Argon+Nitrogen (GII)) and exposure times (1 min and 3 min). Cell viability assays confirmed the non-cytotoxic nature of the plasma-treated medium. Intracellular RNS and ROS levels provided insights into the redox state of the fibroblast cells exposed to media treated with cold plasma-conditioned media (PCM). The ROS/RNS levels in the plasma-treated samples remained comparable with the control fibroblast cells grown in normal media. Malondialdehyde levels, an important biochemical marker of oxidative stress, in the cold plasma treated groups was comparable with the control group, suggesting the favorable modulation of ROS by the cellular antioxidant mechanisms. Accelerated wound-closure rates from 6th hr to 24th hr in all the treated groups ranged from 38.76% to 45.66%, when compared to 34.25% in the control cells. Substantial cell migration leading to 51.59% of wound closure was recorded in the Argon+Nitrogen (GII) group exposed for 3 min. Taken together, the potential of cold plasma to effectively heal wounds without causing prolonged oxidative stress and chronic inflammation is implicated. These outcomes suggest scope for clinical application of C-APPJ as safe and cost-effective treatment of wounds (ulcers, burns, diabetic foot) and wound disinfection.
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Details
- Title
- Biochemical evaluation of wound healing efficacy of cold plasma-conditioned media under different operational conditions
- Creators
- Sonakshi PuriSumit Kumar MandalNavin Kumar SharmaPriti PalRam Prakash LambaVandana MillerUdit Narayan PalDeepa P R
- Publication Details
- Journal of physics. D, Applied physics
- Publisher
- Institute of Physics (IOP)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology Department Internal Research
- Web of Science ID
- WOS:001268875700001
- Scopus ID
- 2-s2.0-85198653552
- Other Identifier
- 991021889791104721
InCites Highlights
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Physics, Applied