Journal article
Biofilm formation by ica-positive and ica-negative strains of Staphylococcus epidermidis in vitro
Biofouling (Chur, Switzerland), v 25(4), pp 367-375
Sep 2010
PMID: 19267282
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Staphylococcus epidermidis is a clinically important opportunistic pathogen that forms biofilm infections on nearly all types of indwelling medical devices. The biofilm forming capability of S. epidermidis has been linked to the presence of the ica operon in the genome, and the amount of biofilm formation measured by the crystal violet (CV) adherence assay. Six S. epidermidis strains were characterized for their ica status using PCR, and their biofilm forming ability over 6 days, using the CV assay and a flow cell system. Ica-negative strains characterized as 'negative for biofilm formation' based on the CV assay were demonstrated to form strongly attached biofilms after 6 days. However, the biofilms were not as extensive as the ica-positive strains. It was concluded that ica is not required for biofilm formation, nor is the 24-h CV assay generalizable for predicting the 6-day biofilm-forming ability for all S. epidermidis strains.
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Details
- Title
- Biofilm formation by ica-positive and ica-negative strains of Staphylococcus epidermidis in vitro
- Creators
- Bethany Dice - Center for Genomic Sciences, Allegheny-Singer Research Institute, Allegheny General HospitalP Stoodley - Department of Microbiology and Immunology , Drexel University College of Medicine Allegheny CampusFarrel Buchinsky - Department of Microbiology and Immunology , Drexel University College of Medicine Allegheny CampusNalini Metha - Center for Genomic Sciences, Allegheny-Singer Research Institute, Allegheny General HospitalGarth D Ehrlich - Department of Bioengineering , Carnegie Mellon UniversityFen Z Hu - Department of Microbiology and Immunology , Drexel University College of Medicine Allegheny Campus
- Publication Details
- Biofouling (Chur, Switzerland), v 25(4), pp 367-375
- Publisher
- Taylor & Francis
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology; Otolaryngology (and Head and Neck Surgery)
- Web of Science ID
- WOS:000264375200006
- Scopus ID
- 2-s2.0-65549150365
- Other Identifier
- 991014877928504721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biotechnology & Applied Microbiology
- Marine & Freshwater Biology