Journal article
Bioinspired coacervate-based bioinks for construction of multiscale tissue engineering scaffolds
Nano research, v 17(9), pp 8209-8219
Sep 2024
Abstract
Engineering hydrogels that resemble biological tissues of various lengths via conventional fabrication techniques remains challenging. Three-dimensional (3D) bioprinting has emerged as an advanced approach for constructing complex biomimetic 3D architectures, which are currently restricted by the limited number of available bioinks with high printability, biomimicry, biocompatibility, and proper mechanical properties. Inspired by ubiquitous coacervation phenomena in biology, we present a unique mineral-biopolymer coacervation strategy that enables the hierarchical assembly of nanoclay and recombinant human collagen (RHC). This system was observed to undergo a coacervation transition (liquid–liquid phase separation) spontaneously. The formed dense phase separated from its supernatant is the coacervate of clay-RHC-rich complexes, where polymer chains are sandwiched between silicate layers. Molecular dynamics simulation was first used to verify and explore the coacervation process. Then, the coacervates were demonstrated to be potential bioinks that exhibited excellent self-supporting and shear-thinning viscoelastic properties. Through extrusion-based printing, the versatility of the bioink was demonstrated by reconstructing the key features of several biological tissues, including multilayered lattice, vascular, nose, and ear-like structures, without the need for precrosslinking operations or support baths. Furthermore, the printed scaffolds were cytocompatible, elicited minimal inflammatory responses, and promoted bone regeneration in calvarial defects.
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Details
- Title
- Bioinspired coacervate-based bioinks for construction of multiscale tissue engineering scaffolds
- Creators
- Zhongwei Guo - Zhengzhou UniversityShiqiang Zhang - Zhengzhou UniversityYilin Guo - Zhengzhou UniversityJingjing Xia - Tsinghua UniversityXiao Wu - Zhengzhou UniversityHao Hu - Sun Yat-sen UniversityRongcheng Hu - Sun Yat-sen UniversityFangli Huang - Sun Yat-sen UniversityQiulei Gao - Zhengzhou UniversityChun Liu - Sun Yat-sen UniversityJingjiang Qiu - Zhengzhou UniversityWei Sun (Corresponding Author) - Drexel University, Mechanical Engineering and Mechanics
- Publication Details
- Nano research, v 17(9), pp 8209-8219
- Publisher
- Springer Nature
- Number of pages
- 11
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:001276181200005
- Scopus ID
- 2-s2.0-85199379681
- Other Identifier
- 991022202109104721