Journal article
Biology of the dopamine transporter on human macrophages
The FASEB journal, v 35(S1), pn/a
May 2021
Abstract
Neuromodulators, such as norepinephrine and dopamine, regulate immune responses. In addition to receptor competency, select immune cells express machinery to engage in active neurotransmitter transmission. Specifically, macrophages express the norepinephrine (NET) and dopamine transporters (DAT). However, how these transporters ‐ classically studied in central neurons ‐ affect immunity remains poorly understood. To this end, we investigated the expression and function of NET and DAT in human macrophages. We found that monocyte‐derived macrophages express functional NET and DAT in vitro. A subset of human intestinal macrophages were further confirmed to express DAT in situ. Interestingly, we discovered that inhibiting DAT, but not NET, enhanced the pro‐inflammatory macrophage program in response to an immune challenge. We attribute this modulation to an observed LPS‐induced efflux of dopamine through DAT that engages an autocrine dopamine signaling loop. Collectively, we propose that DAT actively regulates dopamine signaling on immune cells, and, in this context, DAT on macrophages is a novel, dynamic immunomodulator.
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Details
- Title
- Biology of the dopamine transporter on human macrophages
- Creators
- Phillip Mackie - University of FloridaAdithya Gopinath - University of FloridaDominic Montas - University of FloridaAlyssa Nielsen - University of FloridaMartin Badov - University of FloridaJoshua Riklan - University of FloridaBasil Hashimi - University of FloridaJohn McNamee - University of FloridaRachel Nolan - Drexel UniversityKaitlyn Runner - Drexel UniversityAndria Doty - University of FloridaAdolfo Ramirez-Zamora - University of FloridaPeter Gaskill - Drexel UniversityWolfgang Streit - University of FloridaMichael Okun - University of FloridaHabibeh Khoshbouei - University of Florida
- Publication Details
- The FASEB journal, v 35(S1), pn/a
- Publisher
- The Federation of American Societies for Experimental Biology
- Number of pages
- 1
- Grant note
- University of Florida Clinical and Translational Science (TL1TR001428; UL1TR001427)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- College of Medicine; Pharmacology and Physiology; Drexel University
- Web of Science ID
- WOS:000669893102557
- Other Identifier
- 991020099243904721
InCites Highlights
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- Web of Science research areas
- Biochemistry & Molecular Biology
- Biology
- Cell Biology