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Journal article
Biomechanical properties of murine meniscus surface via AFM-based nanoindentation
Journal of biomechanics, v 48(8), pp 1364-1370
01 Jun 2015
PMCID: PMC4442049
PMID: 25817332
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
This study aimed to quantify the biomechanical properties of murine meniscus surface. Atomic force microscopy (AFM)-based nanoindentation was performed on the central region, proximal side of menisci from 6- to 24-week old male C57BL/6 mice using microspherical tips (Rtip≈5µm) in PBS. A unique, linear correlation between indentation depth, D, and response force, F, was found on menisci from all age groups. This non-Hertzian behavior is likely due to the dominance of tensile resistance by the collagen fibril bundles on meniscus surface that are mostly aligned along the circumferential direction. The indentation resistance was calculated as both the effective modulus, Eind, via the isotropic Hertz model, and the effective stiffness, Sind = dF/dD. Values of Sind and Eind were found to depend on indentation rate, suggesting the existence of poro-viscoelasticity. These values do not significantly vary with anatomical sites, lateral versus medial compartments, or mouse age. In addition, Eind of meniscus surface (e.g., 6.1±0.8MPa for 12 weeks of age, mean±SEM, n=13) was found to be significantly higher than those of meniscus surfaces in other species, and of murine articular cartilage surface (1.4±0.1MPa, n=6). In summary, these results provided the first direct mechanical knowledge of murine knee meniscus tissues. We expect this understanding to serve as a mechanics-based benchmark for further probing the developmental biology and osteoarthritis symptoms of meniscus in various murine models.
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Details
- Title
- Biomechanical properties of murine meniscus surface via AFM-based nanoindentation
- Creators
- Qing Li - Drexel UniversityBasak Doyran - Drexel UniversityLaura W. Gamer - Harvard UniversityX. Lucas Lu - University of DelawareLing Qin - University of PennsylvaniaChristine Ortiz - Massachusetts Institute of TechnologyAlan J. Grodzinsky - Massachusetts Institute of TechnologyVicki Rosen - Harvard UniversityLin Han - School of Biomedical Engineering, Science, and Health Systems, Drexel University, Philadelphia, PA 19104, United States
- Publication Details
- Journal of biomechanics, v 48(8), pp 1364-1370
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000356120000005
- Scopus ID
- 2-s2.0-84929656320
- Other Identifier
- 991019168142504721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biophysics
- Engineering, Biomedical