Journal article
Biomechanics of single zonal chondrocytes
Journal of biomechanics, v 39(9), pp 1595-1602
2006
PMID: 15992803
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Articular cartilage has a distinct zonal architecture, and previous work has shown that chondrocytes from different zones exhibit variations in gene expression and biosynthesis. In this study, the material properties of single chondrocytes from the superficial and middle/deep zones of bovine distal metatarsal articular cartilage were determined using unconfined compression and digital videocapture. To determine the viscoelastic properties of zonal chondrocytes, unconfined creep compression experiments were performed and the resulting creep curves of individual cells were fit using a standard linear viscoelastic solid model. In the model, a fixed value of the Poisson's ratio was used, determined optically from direct compression of middle/deep chondrocytes. The two approaches used in this study yielded the following average material properties of single chondrocytes: Poisson's ratio of 0.26+/-0.08, instantaneous modulus of 1.06+/-0.82 kPa, relaxed modulus of 0.78+/-0.58 kPa, and apparent viscosity of 4.08+/-7.20 kPa s. Superficial zone chondrocytes were found to be significantly stiffer than middle/deep zone chondrocytes. Attachment time did not affect the stiffness of the cells. The zonal variation in viscoelastic properties may result from the distinct mechanical environments experienced by the cells in vivo. Identifying intrinsic differences in the biomechanics of superficial and middle/deep zone chondrocytes is an important component in understanding how biomechanics influence articular cartilage health and disease.
Metrics
Details
- Title
- Biomechanics of single zonal chondrocytes
- Creators
- Adrian C Shieh - Department of Bioengineering, Rice University, MS-142, P.O. Box 1892, Houston, TX 77251-1892, USAKyriacos A Athanasiou
- Publication Details
- Journal of biomechanics, v 39(9), pp 1595-1602
- Publisher
- Elsevier; United States
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000238777500003
- Scopus ID
- 2-s2.0-33744800567
- Other Identifier
- 991014878212804721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biophysics
- Engineering, Biomedical