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Biomimetic proteoglycans can molecularly engineer early osteoarthritic cartilage in vivo
Journal article   Open access   Peer reviewed

Biomimetic proteoglycans can molecularly engineer early osteoarthritic cartilage in vivo

Evan R Phillips, Katsiaryna Prudnikova, Tuan Bui, Adam J Taylor, Diego A Galindo, Richard B Huneke, J. Steve Hou, Mary K Mulcahey and Michele S Marcolongo
Journal of orthopaedic research, v 37(2), pp 403-411
Feb 2019
PMID: 30480335
url
https://doi.org/10.1002/jor.24193View
Published, Version of Record (VoR)Maybe Open Access (Publisher Bronze) Open

Abstract

pericellular cartilage repair biomimetic proteoglycans regeneration osteoarthritis cartilage diffusion
ABSTRACT Biomimetic proteoglycans (BPGs) have the potential to treat osteoarthritis (OA) given that these molecules mimic the structure and properties of natural proteoglycans, which are significantly reduced in OA. We examined the effects of BPGs injected into the intra‐articular space in an in vivo OA rabbit knee model and evaluated the effect on histological response, joint friction, and BPG distribution and retention. Rabbits underwent ACL transection to create an arthritic state after 5 weeks. OA rabbits were treated with BPGs or Euflexxa® (hyaluronic acid) intra‐articular injections. Non‐OA rabbits were injected similarly with BPGs; contralateral joints served as controls. The progression of OA and response to injections were evaluated using Mankin and gross grading systems indicating that mild OA was achieved in operated joints. The coefficient of friction (COF) of the intact knee joints were measured using a custom pendulum friction apparatus, showing that OA joints and OA + Euflexxa® joints demonstrated increased COF than non‐operated controls, while BPG‐injected non‐OA and OA + BPGs were not significantly different from non‐OA controls. Injected fluorescently labeled BPGs demonstrated that BPGs diffused into cartilage with localization in the pericellular region. © 2018 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 37:403–411, 2019.

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Collaboration types
Domestic collaboration
Web of Science research areas
Orthopedics
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