Journal article
Biomimetic proteoglycans diffuse throughout articular cartilage and localize within the pericellular matrix
Journal of biomedical materials research. Part A, v 107(9), pp 1977-1987
01 Sep 2019
PMID: 31056821
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Biomimetic proteoglycan (BPG) diffusion into articular cartilage has the potential to restore the lost proteoglycan content in osteoarthritic cartilage given these molecules mimic the structure and properties of natural proteoglycans. We examined the diffusion characteristics of BPGs through cartilage with the use of a custom-made in vitro cartilage diffusion model in both normal bovine and human osteoarthritic cartilage explants. BPGs were introduced into the cartilage through essentially one-dimensional diffusion using osteochondral plugs. The molecular diffusion was shown to be size and concentration dependent. Diffusion profiles were found over different diffusion time intervals and the profiles were fit to a nonlinear Fickian diffusion model. Steady state 011012-7diffusion coefficients for BPGs were found to be 4.01 and 3.53 mu m(2)/s for 180 and 1600 kDa BPGs, respectfully, and these values are similar to other large molecule diffusion in cartilage. In both bovine and osteoarthritic human cartilage, BPGs were found localized around the chondrocytes. BPG localization was examined by labeling collagen type VI and soaking 5 mu m thick sections of cartilage with BPG solutions demonstrating that the BPGs diffused into the cartilage and preferentially localized alongside collagen type VI in the pericellular matrix.
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Details
- Title
- Biomimetic proteoglycans diffuse throughout articular cartilage and localize within the pericellular matrix
- Creators
- Evan R. Phillips - Drexel UniversityBrett D. Haislup - Drexel UniversityNicholas Bertha - Drexel UniversityMaria Lefchak - Drexel UniversityJoseph Sincavage - Drexel UniversityKatsiaryna Prudnikova - Drexel UniversityBrandon Shallop - Hahnemann University HospitalMary K. Mulcahey - Tulane UniversityMichele S. Marcolongo - Drexel University
- Publication Details
- Journal of biomedical materials research. Part A, v 107(9), pp 1977-1987
- Publisher
- Wiley
- Number of pages
- 11
- Grant note
- CMMI 1826202 / National Science Foundation; National Science Foundation (NSF) CURE Grant State of Pennsylvania Clinical & Translational Research Institute Drexel University
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Materials Science and Engineering; Orthopedic/Orthopaedic Surgery
- Web of Science ID
- WOS:000475476600012
- Scopus ID
- 2-s2.0-85066898256
- Other Identifier
- 991019169583104721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Engineering, Biomedical
- Materials Science, Biomaterials