Journal article
Bioprinted nanoparticles for tissue engineering applications
Tissue engineering. Part C, Methods, v 16(4), pp 631-642
Aug 2010
PMID: 19769526
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Tissue engineering may require precise patterning and monitoring of cells and bioactive factors within the scaffold. We investigated a new hybrid nanobioprinting technique that facilitates manipulation and tracking of cells and bioactive factors within a three-dimensional tissue construct. This technique combines the initial patterning capabilities of syringe-based cell deposition with the active patterning capabilities of superparamagnetic nanoparticles. Superparamagnetic iron oxide nanoparticles, either in the alginate biopolymer or loaded inside endothelial cells, were bioprinted using a solid freeform fabrication direct cell writing system. Bioprinting did not impact cell viability when nanoparticles were in the alginate. However, both control and printed samples with 0.1 or 1.0 mg/mL nanoparticles in the alginate showed a 16% or 35% viability loss at 36 h after printing, respectively. Nanoparticle loading in cells decreased cell viability to 11% and bioprinting decreased viability to an additional 29% at 36 h. No changes were observed in any samples after 36 h, suggesting that cell viability stabilized following the initial nanoparticle toxicity effect. Nanoparticles in the alginate and those loaded in cells were moved using an external magnet, depending on biopolymer viscosity, and imaged by microcomputed tomography. The hybrid nanobioprinting method can noninvasively manipulate and track bioactive factors and cells within tissue engineering structures.
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Details
- Title
- Bioprinted nanoparticles for tissue engineering applications
- Creators
- Kivilcim Buyukhatipoglu - Drexel UniversityRobert ChangWei SunAlisa Morss Clyne
- Publication Details
- Tissue engineering. Part C, Methods, v 16(4), pp 631-642
- Publisher
- Mary Ann Liebert
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:000280228300008
- Scopus ID
- 2-s2.0-77954960401
- Other Identifier
- 991019167416304721
UN Sustainable Development Goals (SDGs)
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Cell & Tissue Engineering
- Cell Biology
- Engineering, Biomedical
- Materials Science, Biomaterials