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Accepted (AM)Open Access (License Unspecified), Open
Journal article
Bioprinting of 3D breast epithelial spheroids for human cancer models
Biofabrication, v 11(2), pp 025003/1-025003/11
01 Apr 2019
PMID: 30616234
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
3D human cancer models provide a better platform for drug efficacy studies than conventional 2D culture, since they recapitulate important aspects of the in vivo microenvironment. While biofabrication has advanced model creation, bioprinting generally involves extruding individual cells in a bioink and then waiting for these cells to self-assemble into a hierarchical 3D tissue. This self-assembly is time consuming and requires complex cellular interactions with other cell types, extracellular matrix components, and growth factors. We therefore investigated if we could directly bioprint pre-formed 3D spheroids in alginate-based bioinks to create a model tissue that could be used almost immediately. Human breast epithelial cell lines were bioprinted as individual cells or as preformed spheroids, either in monoculture or co-culture with vascular endothelial cells. While individual breast cells only spontaneously formed spheroids in Matrigel-based bioink, pre-formed breast spheroids maintained their viability, architecture, and function after bioprinting. Bioprinted breast spheroids were more resistant to paclitaxel than individually printed breast cells; however, this effect was abrogated by endothelial cell co-culture. This study shows that 3D cellular structure bioprinting has potential to create tissue models that quickly replicate the tumor microenvironment.
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Details
- Title
- Bioprinting of 3D breast epithelial spheroids for human cancer models
- Creators
- Swathi Swaminathan - Drexel UniversityQudus Hamid - Drexel Univ, Dept Mech Engn & Mech, Philadelphia, PA 19104 USAWei Sun - Drexel UniversityAlisa Morss Clyne - Drexel Univ, Dept Mech Engn & Mech, Philadelphia, PA 19104 USA
- Publication Details
- Biofabrication, v 11(2), pp 025003/1-025003/11
- Publisher
- Iop Publishing Ltd
- Number of pages
- 11
- Grant note
- R01 HL140239 / NHLBI NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Heart Lung & Blood Institute (NHLBI)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology; Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:000456874600001
- Scopus ID
- 2-s2.0-85060513211
- Other Identifier
- 991019167655904721
UN Sustainable Development Goals (SDGs)
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Engineering, Biomedical
- Materials Science, Biomaterials