Journal article
Bioprinting of patient-derived heterogeneous renal cell carcinoma organoids for personalized therapy
Biofabrication, v 17(4), 045008
01 Oct 2025
PMID: 40791084
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Tumor organoids that can accurately recapitulate the pathophysiological characteristics of original tumor are urgently needed for personalized therapy. However, there are few published studies on patient-derived renal cell carcinoma (RCC) heterogeneous organoids for drug testing to account for patient-specific heterogeneous clinical responses, which has significantly impeded research in the field. Traditional RCC organoid technologies involving matrigel droplets require intensive manual manipulation and are hampered by variability, functional immaturity, low throughput, and limited scale. Here, we applied extrusion-based high-throughput bioprinter to rapidly generate heterogeneous RCC organoids with uniform size, realizing batch automated stable construction and quality control. Bioprinted RCC organoids reserved the pathological morphology and gene mutation/expression characteristics of original tumor and demonstrate interorganoid and interpatient heterogeneity even after long-term cultivation, which are suitable for preclinical patient-specific drug screening testing. Finally, we created multicellular assembloids by reconstituting RCC aggregates with stromal components to generate an organized architecture with in vivo-like vascular morphology and spatial tumor microenvironment heterogeneity. Thus, we have demonstrated the wide-ranging biomedical utility of bioprinted organoids in furthering our understanding of the physiological mechanisms of tumors and the development of personalized treatment methods.
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Details
- Title
- Bioprinting of patient-derived heterogeneous renal cell carcinoma organoids for personalized therapy
- Creators
- Shuangshuang Mao - Tsinghua UniversityRuiyang Xie - Peking UniversityJianzhong Shou - Chinese Academy of Medical Sciences & Peking Union Medical CollegeYuan Pang - Tsinghua UniversityWei Sun - Drexel University
- Publication Details
- Biofabrication, v 17(4), 045008
- Publisher
- IOP Publishing
- Number of pages
- 21
- Grant note
- 20220484075 / Beijing Nova Program; Beijing Municipal Science & Technology Commission 100304002 / Tsinghua Mechanical Engineering Department Research Fund NPKF-2023-02 / NMPA Key Laboratory Research Fund 52175273; 52422508 / National Natural Science Foundation of China; National Natural Science Foundation of China (NSFC) 2022ZLB004 / Tsinghua University Institute for Intelligent Healthcare Program; Tsinghua University RS2024H006 / Key Project of National Medical Products Administration B17026 / The 111 Project; Ministry of Education, China - 111 Project Z23110000 7223006 / Beijing Science and Technology Plan 2022NSCQ-LZX0326 / Natural Science Foundation of Chongqing 7254443 / Beijing Municipal Natural Science Foundation; Beijing Natural Science Foundation
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- [Retired Faculty]; Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:001548180700001
- Scopus ID
- 2-s2.0-105013344949
- Other Identifier
- 991022197411404721
UN Sustainable Development Goals (SDGs)
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Engineering, Biomedical
- Materials Science, Biomaterials