Journal article
Bioprinting of patient-derived in vitro intrahepatic cholangiocarcinoma tumor model: establishment, evaluation and anti-cancer drug testing
Biofabrication, v 12(4), pp 045014/1-045014/15
Oct 2020
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Towards the development of in vivo-mimicking tumor model for extensive study of tumorigenesis and establishment of personalized therapy, patient-derived primary tumor cells were employed in this work for three-dimensional (3D) bioprinting. Intrahepatic cholangiocarcinoma cells isolated from patient were bioprinted using a composite hydrogel system of gelatin-alginate-MatrigelTM into pre-designed grid architecture. ICC cells were observed to process a colony forming ability with high survival rate and active proliferation. Expression levels of tumor markers, cancer stem cell markers, matrix metalloproteinase protein, index of tumor fibrosis, index of liver function, and epithelial-mesenchymal transition regulatory proteins confirmed the development of the invasive and metastatic phenotype of the intrahepatic cholangiocarcinoma cells in the 3D printed tumor microenvironment. Similar results were obtained in anti-cancer drug resistance of the intrahepatic cholangiocarcinoma cells in the 3D bioprinted construct that demonstrated stem-like properties, which suggested the promising potential of current 3D printed tumor model in the development of personalized therapy, especially for discovery of more conducive targeted drugs.
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Details
- Title
- Bioprinting of patient-derived in vitro intrahepatic cholangiocarcinoma tumor model: establishment, evaluation and anti-cancer drug testing
- Creators
- Shuangshuang Mao - Tsinghua UniversityJianyu He - Tsinghua UniversityYu Zhao - Tsinghua UniversityTiankun Liu - Tsinghua UniversityFeihu Xie - Peking Union Medical College HospitalHuayu Yang - Peking Union Medical College HospitalYilei Mao - Peking Union Medical College HospitalYuan Pang - Tsinghua UniversityWei Sun - Tsinghua University
- Publication Details
- Biofabrication, v 12(4), pp 045014/1-045014/15
- Publisher
- IOP Publishing
- Number of pages
- 15
- Grant note
- 3184049 / Beijing Natural Science Foundation 51805294 / National Natural Science Foundation of China (http://dx.doi.org/10.13039/501100001809) XDA15014300 / Strategic Priority Program (SPP) on Space Science B17026 / 111 Project 2019Z08QCX14 / Independent Scientific Research Program of Tsinghua University
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:000556779900001
- Scopus ID
- 2-s2.0-85088881297
- Other Identifier
- 991019167419504721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Engineering, Biomedical
- Materials Science, Biomaterials