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Journal article
Bioprinting three-dimensional cell-laden tissue constructs with controllable degradation
Scientific reports, v 6(1), pp 24474-24474
19 Apr 2016
PMID: 27091175
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Alginate hydrogel is a popular biologically inert material that is widely used in 3D bioprinting, especially in extrusion-based printing. However, the printed cells in this hydrogel could not degrade the surrounding alginate gel matrix, causing them to remain in a poorly proliferating and non-differentiating state. Here, we report a novel study of the 3D printing of human corneal epithelial cells (HCECs)/collagen/gelatin/alginate hydrogel incubated with a medium containing sodium citrate to obtain degradation-controllable cell-laden tissue constructs. The 3D-printed hydrogel network with interconnected channels and a macroporous structure was stable and achieved high cell viability (over 90%). By altering the mole ratio of sodium citrate/sodium alginate, the degradation time of the bioprinting constructs can be controlled. Cell proliferation and specific marker protein expression results also revealed that with the help of sodium citrate degradation, the printed HCECs showed a higher proliferation rate and greater cytokeratin 3(CK3) expression, indicating that this newly developed method may help to improve the alginate bioink system for the application of 3D bioprinting in tissue engineering.
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Details
- Title
- Bioprinting three-dimensional cell-laden tissue constructs with controllable degradation
- Creators
- Zhengjie Wu - Tsinghua UniversityXin Su - Tsinghua UniversityYuanyuan Xu - Tsinghua UniversityBin Kong - Tsinghua UniversityWei Sun - Tsinghua UniversityShengli Mi - Tsinghua University
- Publication Details
- Scientific reports, v 6(1), pp 24474-24474
- Publisher
- Nature Publishing Group
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:000374353000001
- Scopus ID
- 2-s2.0-84973370305
- Other Identifier
- 991019167456804721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Materials Science, Biomaterials