Journal article
Biosensor-based microRNA detection: techniques, design, performance, and challenges
Analyst (London), v 139(7), pp 1576-1588
07 Apr 2014
PMID: 24501736
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The current state of biosensor-based techniques for amplification-free microRNA (miRNA) detection is critically reviewed. Comparison with non-sensor and amplification-based molecular techniques (MTs), such as polymerase-based methods, is made in terms of transduction mechanism, associated protocol, and sensitivity. Challenges associated with miRNA hybridization thermodynamics which affect assay selectivity and amplification bias are briefly discussed. Electrochemical, electromechanical, and optical classes of miRNA biosensors are reviewed in terms of transduction mechanism, limit of detection (LOD), time-to-results (TTR), multiplexing potential, and measurement robustness. Current trends suggest that biosensor-based techniques (BTs) for miRNA assay will complement MTs due to the advantages of amplification-free detection, LOD being femtomolar (fM)-attomolar (aM), short TTR, multiplexing capability, and minimal sample preparation requirement. Areas of future importance in miRNA BT development are presented which include focus on achieving high measurement confidence and multiplexing capabilities.
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Details
- Title
- Biosensor-based microRNA detection: techniques, design, performance, and challenges
- Creators
- Blake N Johnson - Department of Chemical and Biological Engineering, Drexel University, Philadelphia, PA 19104, USA. mutharasan@drexel.eduRaj Mutharasan
- Publication Details
- Analyst (London), v 139(7), pp 1576-1588
- Publisher
- Royal Society of Chemistry; England
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemical and Biological Engineering
- Web of Science ID
- WOS:000332422400001
- Scopus ID
- 2-s2.0-84897699393
- Other Identifier
- 991014877901904721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Chemistry, Analytical