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Accepted (AM)Open Access (License Unspecified), Open
Journal article
Bone tissue engineering in a rotating bioreactor using a microcarrier matrix system
Journal of biomedical materials research, v 55(2), pp 242-253
01 May 2001
PMID: 11255176
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
A novel approach was utilized to grow in vitro mineralized bone tissue using lighter-than-water, polymeric scaffolds in a high aspect ratio rotating bioreactor. We have adapted polymer microencapsulation methods for the formation of hollow, lighter-than-water microcarriers of degradable poly(lactic-co-glycolic acid). Scaffolds were fabricated by sintering together lighter-than-water microcarriers from 500 to 860 μm in diameter to create a fully interconnected, three-dimensional network with an average pore size of 187 μm and aggregate density of 0.65 g/mL. Motion in the rotating bioreactor was characterized by numerical simulation and by direct measurement using an in situ particle tracking system. Scaffold constructs established a near circular trajectory in the fluid medium with a terminal velocity of 98 mm/s while avoiding collision with the bioreactor wall. Preliminary cell culture studies on these scaffolds show that osteoblast-like cells readily attached to microcarrier scaffolds using controlled seeding conditions with an average cell density of 6.5 × 104 cells/cm2. The maximum shear stress imparted to attached cells was estimated to be 3.9 dynes/cm2. In addition, cells cultured in vitro on these lighter-than-water scaffolds retained their osteoblastic phenotype and showed significant increases in alkaline phosphatase expression and alizarin red staining by day 7 as compared with statically cultured controls.
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Details
- Title
- Bone tissue engineering in a rotating bioreactor using a microcarrier matrix system
- Creators
- E. A. Botchwey - Drexel UniversityS. R. Pollack - University of PennsylvaniaE. M. Levine - The Wistar InstituteC. T. Laurencin - Drexel University
- Publication Details
- Journal of biomedical materials research, v 55(2), pp 242-253
- Publisher
- Wiley
- Number of pages
- 12
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemical and Biological Engineering
- Web of Science ID
- WOS:000167221200012
- Scopus ID
- 2-s2.0-0035089668
- Other Identifier
- 991019339694504721
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InCites Highlights
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Engineering, Biomedical
- Materials Science, Biomaterials