Journal article
Bradykinin Activates a Phospholipase D that Hydrolyzes Phosphatidylcholine in PC12 Cells
Journal of neurochemistry, v 56(2), pp 509-517
Feb 1991
PMID: 1988554
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Abstract
: In PC12 pheochromocytoma cells whose phospholipids had been prelabeled with [3H]palmitic acid, bradykinin increased the production of [3H]phosphatidic acid. The increase in [3H]phosphatidic acid occurred within 1–2 min, before the majority of the increase in [3H]diacylglycerol. When the phospholipids were prelabeled with [3H]choline, bradykinin increased the intracellular release of [3H]choline. The production of phosphatidic acid and choline suggests that bradykinin was increasing the activity of phospholipase D. Transphosphatidylation is a unique property of phospholipase D. In cells labeled with [3H]palmitic acid, bradykinin stimulated the transfer of phosphatidyl groups to both ethanol and propanol to form [3H]phosphatidylethanol and [3H]phosphatidylpropanol, respectively. The effect of bradykinin on [3H]phosphatidic acid and [3H]phosphatidylethanol formation was partially dependent on extracellular Ca2+. In cells treated with nerve growth factor, carbachol also increased [3H]phosphatidylethanol formation. To investigate the substrate specificity of phospholipase D, cells were labeled with [14C]stearic acid and [3H]palmitic acid, and then incubated with ethanol in the absence or presence of bradykinin. The 14C/3H ratio of the phosphatidylethanol that accumulated in response to bradykinin was almost identical to the 14C/3H ratio of phosphatidylcholine. The 14C/3H ratio in phosphatidic acid and diacylglycerol was higher than the ratio in phosphatidylcholine. These data provide additional support for the idea that bradykinin activates a phospholipase D that is active against phosphatidylcholine. The hydrolysis of phosphatidylcholine by phospholipase D accounts for only a portion of the phosphatidic acid and diacylglycerol that accumulates in bradykinin‐stimulated cells; bradykinin evidently stimulates several pathways of phospholipid metabolism in PC12 cells.
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Details
- Title
- Bradykinin Activates a Phospholipase D that Hydrolyzes Phosphatidylcholine in PC12 Cells
- Creators
- Joel Horwitz - University of Chicago
- Publication Details
- Journal of neurochemistry, v 56(2), pp 509-517
- Publisher
- Blackwell Publishing Ltd
- Number of pages
- 9
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:A1991ET73200020
- Scopus ID
- 2-s2.0-0026058020
- Other Identifier
- 991020532109304721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemistry & Molecular Biology
- Neurosciences