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Brain region-dependent alterations in polysialic acid immunoreactivity across the estrous cycle in mice
Journal article   Open access   Peer reviewed

Brain region-dependent alterations in polysialic acid immunoreactivity across the estrous cycle in mice

Laura L Giacometti, Fangyi Huang, Brianna S Hamilton and Jacqueline M Barker
Hormones and behavior, v 126, 104851
Nov 2020
DOI: https://doi.org/10.1016/j.yhbeh.2020.104851
PMID: 32941849
Featured in Collection :   UN Sustainable Development Goals @ Drexel
url
https://europepmc.org/articles/pmc7725886View
Accepted (AM)Open Access (License Unspecified),  Open

Abstract

Animals Brain - anatomy & histology Brain - metabolism Estrous Cycle - metabolism Estrous Cycle - physiology Female Glycosylation Hippocampus - metabolism Mice Mice, Inbred C57BL Nucleus Accumbens - metabolism Organ Specificity Prefrontal Cortex - metabolism Protein Processing, Post-Translational - physiology Sialic Acids - metabolism Immunohistochemistry
N-glycosylation is a posttranslational modification that plays significant roles in regulating protein function. One form of N-glycosylation, polysialylation, has been implicated in many processes including learning and memory, addiction, and neurodegenerative disease. Polysialylation appears to be modulated by the estrous cycle in the hypothalamus in rat, but this has not been assessed in other brain regions. To determine if polysialylation was similarly estrous phase-dependent in other neuroanatomical structures, the percent area of polysialic acid (PSA) immunoreactivity in subregions of the medial prefrontal cortex, hippocampus, and nucleus accumbens was assessed in each of the four phases in adult female mice. In this study, we found that PSA immunoreactivity fluctuated across the estrous cycle in a subregion-specific manner. In the prefrontal cortex, PSA immunoreactivity was significantly lower in proestrus phase compared to estrus in the prelimbic cortex, but did not differ across the estrous cycle in the infralimbic cortex. In the hippocampus, PSA immunoreactivity was significantly increased in proestrus compared to metestrus in the CA1 and CA2 and compared to diestrus in CA3, but remain unchanged in the dentate gyrus. PSA immunoreactivity did not vary across the estrous cycle in the nucleus accumbens core or shell. These findings may have implications for estrous cycle-dependent alterations in behavior.

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Web of Science research areas
Behavioral Sciences
Endocrinology & Metabolism
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