Journal article
Brain-wide cellular resolution imaging of Cre transgenic zebrafish lines for functional circuit-mapping
eLife, v 8
08 Feb 2019
PMID: 30735129
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Decoding the functional connectivity of the nervous system is facilitated by transgenic methods that express a genetically encoded reporter or effector in specific neurons; however, most transgenic lines show broad spatiotemporal and cell-type expression. Increased specificity can be achieved using intersectional genetic methods which restrict reporter expression to cells that co-express multiple drivers, such as Gal4 and Cre. To facilitate intersectional targeting in zebrafish, we have generated more than 50 new Cre lines, and co-registered brain expression images with the Zebrafish Brain Browser, a cellular resolution atlas of 264 transgenic lines. Lines labeling neurons of interest can be identified using a web-browser to perform a 3D spatial search (zbbrowser.com). This resource facilitates the design of intersectional genetic experiments and will advance a wide range of precision circuit-mapping studies.
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Details
- Title
- Brain-wide cellular resolution imaging of Cre transgenic zebrafish lines for functional circuit-mapping
- Creators
- Kathryn M Tabor - Eunice Kennedy Shriver National Institute of Child Health and Human DevelopmentGregory D Marquart - Eunice Kennedy Shriver National Institute of Child Health and Human DevelopmentChristopher Hurt - Virginia TechTrevor S Smith - Eunice Kennedy Shriver National Institute of Child Health and Human DevelopmentAlexandra K Geoca - Eunice Kennedy Shriver National Institute of Child Health and Human DevelopmentAshwin A Bhandiwad - Eunice Kennedy Shriver National Institute of Child Health and Human DevelopmentAbhignya Subedi - National Institute of General Medical SciencesJennifer L Sinclair - Eunice Kennedy Shriver National Institute of Child Health and Human DevelopmentHannah M Rose - Eunice Kennedy Shriver National Institute of Child Health and Human DevelopmentNicholas F Polys - Virginia TechHarold A Burgess - Eunice Kennedy Shriver National Institute of Child Health and Human Development
- Publication Details
- eLife, v 8
- Publisher
- eLife
- Grant note
- 1ZIAHD008884-04 / Eunice Kennedy Shriver National Institute of Child Health and Human Development Advanced Research Computing / Virginia Tech NA / Virginia Tech Advanced Research Computing
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000459923200001
- Scopus ID
- 2-s2.0-85063613483
- Other Identifier
- 991020111031704721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biology