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Journal article
C53 Interacting with UFM1-Protein Ligase 1 Regulates Microtubule Nucleation in Response to ER Stress
Cells (Basel, Switzerland), v 11(3), 555
05 Feb 2022
PMID: 35159364
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
ER distribution depends on microtubules, and ER homeostasis disturbance activates the unfolded protein response resulting in ER remodeling. CDK5RAP3 (C53) implicated in various signaling pathways interacts with UFM1-protein ligase 1 (UFL1), which mediates the ufmylation of proteins in response to ER stress. Here we find that UFL1 and C53 associate with γ-tubulin ring complex proteins. Knockout of UFL1 or C53 in human osteosarcoma cells induces ER stress and boosts centrosomal microtubule nucleation accompanied by γ-tubulin accumulation, microtubule formation, and ER expansion. C53, which is stabilized by UFL1, associates with the centrosome and rescues microtubule nucleation in cells lacking UFL1. Pharmacological induction of ER stress by tunicamycin also leads to increased microtubule nucleation and ER expansion. Furthermore, tunicamycin suppresses the association of C53 with the centrosome. These findings point to a novel mechanism for the relief of ER stress by stimulation of centrosomal microtubule nucleation.
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Details
- Title
- C53 Interacting with UFM1-Protein Ligase 1 Regulates Microtubule Nucleation in Response to ER Stress
- Creators
- Anastasiya Klebanovych - Czech Academy of Sciences, Institute of Molecular GeneticsStanislav Vinopal - Cytoskeleton (United States)Eduarda Dráberová - Czech Academy of SciencesVladimíra SládkováTetyana SulimenkoVadym Sulimenko - Cytoskeleton (United States)Věra Vosecká - Czech Academy of Sciences, Institute of Molecular GeneticsLibor Macůrek - Czech Academy of Sciences, Institute of Molecular GeneticsAgustin Legido - Drexel University, PediatricsPavel Dráber - Cytoskeleton (United States)
- Publication Details
- Cells (Basel, Switzerland), v 11(3), 555
- Publisher
- MDPI
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pediatrics
- Web of Science ID
- WOS:000761016100001
- Scopus ID
- 2-s2.0-85124364476
- Other Identifier
- 991021860720604721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Cell Biology