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Journal article
CADM1 is a TWIST1-regulated suppressor of invasion and survival
Cell death & disease, v 10(4), pp 281-281
25 Mar 2019
PMID: 30911007
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Metastatic cancer remains a clinical challenge; however, patients diagnosed prior to metastatic dissemination have a good prognosis. The transcription factor, TWIST1 has been implicated in enhancing the migration and invasion steps within the metastatic cascade, but the range of TWIST1-regulated targets is poorly described. In this study, we performed expression profiling to identify the TWIST1-regulated transcriptome of melanoma cells. Gene ontology pathway analysis revealed that TWIST1 and epithelial to mesenchymal transition (EMT) were inversely correlated with levels of cell adhesion molecule 1 (CADM1). Chromatin immunoprecipitation (ChIP) studies and promoter assays demonstrated that TWIST1 physically interacts with the CADM1 promoter, suggesting TWIST1 directly represses CADM1 levels. Increased expression of CADM1 resulted in significant inhibition of motility and invasiveness of melanoma cells. In addition, elevated CADM1 elicited caspase-independent cell death in non-adherent conditions. Expression array analysis suggests that CADM1 directed non-adherent cell death is associated with loss of mitochondrial membrane potential and subsequent failure of oxidative phosphorylation pathways. Importantly, tissue microarray analysis and clinical data from TCGA indicate that CADM1 expression is inversely associated with melanoma progression and positively correlated with better overall survival in patients. Together, these data suggest that CADM1 exerts tumor suppressive functions in melanoma by reducing invasive potential and may be considered a biomarker for favorable prognosis.
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Details
- Title
- CADM1 is a TWIST1-regulated suppressor of invasion and survival
- Creators
- Edward J. Hartsough - Drexel UniversityMichele B. Weiss - Thomas Jefferson UniversityShea A. Heilman - Thomas Jefferson UniversityTimothy J. Purwin - Thomas Jefferson UniversityCurtis H. Kugel - Thomas Jefferson UniversitySheera R. Rosenbaum - Thomas Jefferson UniversityDan A. Erkes - Thomas Jefferson UniversityManoela Tiago - Thomas Jefferson UniversityKim HooKim - Thomas Jefferson UniversityInna Chervoneva - Thomas Jefferson UniversityAndrew E. Aplin - Thomas Jefferson University
- Publication Details
- Cell death & disease, v 10(4), pp 281-281
- Publisher
- Springer Nature
- Number of pages
- 15
- Grant note
- PF-18-096-01-LIB / American Cancer Society-CEO's Against Cancer-PA Chapter Postdoctoral Fellowship P30 CA056036 / NIH/National Cancer Institute; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI) K99/R00 CA207855; R01 CA182635; R01 CA196278 / National Cancer Center and National Institutes of Health (NIH) R01CA196278 / NATIONAL CANCER INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:000462415100002
- Scopus ID
- 2-s2.0-85063587919
- Other Identifier
- 991019168807304721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Cell Biology