Journal article
CD28 Costimulation Is Required for the Expression of T-Cell-Dependent Cell-Mediated Immunity against Blood-Stage Plasmodium chabaudi Malaria Parasites
Infection and immunity, Vol.72(10), pp.5768-5774
Oct 2004
PMCID: PMC517583
PMID: 15385476
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Mice suppress the parasitemia of acute blood-stage
Plasmodium chabaudi
malaria by an antibody- or T-cell-dependent cell-mediated mechanism of immunity (AMI and CMI, respectively) or by both mechanisms. To determine whether CD28 costimulation is required for expression of these polar immune responses, we first compared the time courses of
P. chabaudi
malaria in CD28-deficient (CD28
−/−
) and CD28-intact (CD28
+/+
) mice. Acute infections in both knockout (KO) and control mice followed similar time courses, with the period of descending parasitemia being prolonged ∼2 weeks in KO mice followed by intermittent low-grade chronic parasitemia. Infected CD28
−/−
mice produced primarily the immunoglobulin M antibody, which upon passive transfer provided partial protection against
P. chabaudi
challenge, suggesting that the elimination of blood-stage parasites by CD28
−/−
mice was achieved by AMI. To determine whether CD28
−/−
costimulation is required for the expression of CMI against the parasite, we compared the time courses of parasitemia in B-cell-deficient double-KO (J
H
−/−
× CD28
−/−
) mice and control (J
H
−/−
× CD28
+/+
) mice. Whereas control mice suppressed parasitemia to subpatent levels within ∼2 weeks postinoculation, double-KO mice developed high levels of parasitemia of long-lasting duration. Although not required for the suppression of acute
P. chabaudi
parasitemia by AMI, CD28 costimulation is essential for the elimination of blood-stage parasites by CMI.
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Details
- Title
- CD28 Costimulation Is Required for the Expression of T-Cell-Dependent Cell-Mediated Immunity against Blood-Stage Plasmodium chabaudi Malaria Parasites
- Creators
- Thomas Rummel - Department of Medical Microbiology and Immunology, University of Wisconsin Medical School, Madison, WisconsinJoan Batchelder - Department of Medical Microbiology and Immunology, University of Wisconsin Medical School, Madison, WisconsinPatrick Flaherty - Department of Medical Microbiology and Immunology, University of Wisconsin Medical School, Madison, WisconsinGayeLyn LaFleur - Department of Medical Microbiology and Immunology, University of Wisconsin Medical School, Madison, WisconsinPayal Nanavati - Department of Medical Microbiology and Immunology, University of Wisconsin Medical School, Madison, WisconsinJames M Burns - Department of Medical Microbiology and Immunology, University of Wisconsin Medical School, Madison, WisconsinWilliam P Weidanz - Department of Medical Microbiology and Immunology, University of Wisconsin Medical School, Madison, Wisconsin
- Publication Details
- Infection and immunity, Vol.72(10), pp.5768-5774
- Publisher
- American Society for Microbiology
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Identifiers
- 991014878410804721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Immunology
- Infectious Diseases