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CD9-mediated costimulation of TCR-triggered naive T cells leads to activation followed by apoptosis
Journal article   Open access   Peer reviewed

CD9-mediated costimulation of TCR-triggered naive T cells leads to activation followed by apoptosis

X G Tai, K Toyooka, Y Yashiro, R Abe, C S Park, T Hamaoka, M Kobayashi, S Neben and H Fujiwara
The Journal of immunology (1950), v 159(8), pp 3799-3807
15 Oct 1997
PMID: 9378967
url
https://journals.aai.org/jimmunol/article-pdf/159/8/3799/1076340/3799.pdfView
Published, Version of Record (VoR) Open
url
https://doi.org/10.4049/jimmunol.159.8.3799View
Published, Version of Record (VoR) Open

Abstract

Immunology Life Sciences & Biomedicine Science & Technology
The induction of full activation or death in TCR-triggered T cells depends largely on whether appropriate costimulatory signals are provided. In this study, we show that the costimulation of CD9 on naive T cells during TCR stimulation results in transient, albeit potent, activation followed by apoptosis, rather than full activation. Anti-CD9 mAb synergized with suboptimal doses of anti-CD3 mAb in inducing T cell activation. [H-3]TdR incorporation determined 2 days after CD9 costimulation was as potent as that induced by CD28 costimulation. In contrast to progressive T cell proliferation induced by CD28 costimulation, CD9 costimulation led to the induction of apoptosis of once-activated T cells. Although IL-2R expression was induced significantly earlier and to a greater degree after CD9 costimulation than after CD28 costimulation, CD9 costimulation only transiently produced a small amount of IL-2 and induced apparently low levels of bcl-x(L) compared with those observed in CD28 costimulation. Addition of rIL-2 to cultures of CD9 costimulation induced strikingly enhanced expression of bcl proteins, especially of bcl-x(L), and protected TCR-stimulated T cells from apoptosis. These data indicate that CD9-mediated costimulation of TCR-triggered naive T cells leads to activation followed by apoptosis as the result of failure to generate a positive signal for sufficient levels of IL-2 production.

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