Journal article
CDK phosphorylation of Drc1 regulates DNA replication in fission yeast
Current biology, v 12(7), pp 599-605
02 Apr 2002
PMID: 11937031
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Cyclin-dependent kinases (CDKs) are absolutely required for DNA replication in eukaryotic cells. CDKs are thought to activate one or more replication factors, but the identities of these proteins are unknown. Here we describe fission yeast Drc1, a protein required for DNA replication that is phosphorylated by Cdc2. Drc1 depletion leads to catastrophic mitotic divisions with incompletely replicated DNA, indicating that Drc1 is required for DNA synthesis and S-M replication checkpoint control. Drc1 associates with Cdc2 and is phosphorylated at the onset of S phase when Cdc2 is activated. Mutant Drc1 that lacks CDK phosphorylation sites is nonfunctional and fails to interact with Cut5 replication factor. These data suggest that Cdc2 promotes DNA replication by phosphorylating Drc1 and regulating its association with Cut5.
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Details
- Title
- CDK phosphorylation of Drc1 regulates DNA replication in fission yeast
- Creators
- Eishi Noguchi - Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USAPaul ShanahanChiaki NoguchiPaul Russell
- Publication Details
- Current biology, v 12(7), pp 599-605
- Publisher
- Elsevier; England
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000174953600027
- Scopus ID
- 2-s2.0-0037006806
- Other Identifier
- 991014878525804721
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InCites Highlights
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- Web of Science research areas
- Biochemistry & Molecular Biology
- Biology
- Cell Biology