CK5 is more sensitive than CK5/6 in identifying the "basal-like" phenotype of breast carcinoma

Rohit Bhargava; Sushil Beriwal; Kim McManus; David J Dabbs

doi:10.1309/AJCP3KFF1LTYWQIY

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CK5 is more sensitive than CK5/6 in identifying the "basal-like" phenotype of breast carcinoma

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CK5 is more sensitive than CK5/6 in identifying the "basal-like" phenotype of breast carcinoma

Rohit Bhargava, Sushil Beriwal, Kim McManus and David J Dabbs

American journal of clinical pathology, v 130(5), pp 724-730

01 Nov 2008

DOI: https://doi.org/10.1309/AJCP3KFF1LTYWQIY

PMID: 18854264

Featured in Collection : UN Sustainable Development Goals @ Drexel

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Abstract

Adult

Breast Neoplasms - diagnosis

Breast Neoplasms - pathology

Carcinoma, Basal Cell - diagnosis

Carcinoma, Basal Cell - pathology

Female

Gene Expression Profiling - methods

Humans

Keratin-5 - analysis

Keratin-5 - immunology

Keratin-6 - analysis

Keratin-6 - immunology

Sensitivity and Specificity

Multiple immunohistochemical stains, including cytokeratin (CK)5/6, are used in a panel format to identify "basal-like" carcinomas. We set out to determine the sensitivity and specificity of the CK5 antibody (clone XM26) and compared its expression with that of CK5/6 (clone D5/16B4) in a variety of breast carcinoma cases. The study was performed on 3 breast carcinoma tissue microarrays (TMAs). TMA-1 consisted of 59 consecutive breast carcinoma cases. TMA-2 (n = 16) and TMA-3 (n = 11) consisted of basal-like breast carcinomas previously characterized morphologically and immunohistochemically at our institution. Of the 86 total cases, 20 were positive for CK5 and CK5/6, 14 were positive for CK5 only, and 52 were negative for both. The sensitivity of CK5 for identifying basal-like tumors was 97% compared with 59% for CK5/6. Both antibodies had comparable specificity of more than 95%. For positive cases, the percentage and intensity of staining was much higher with CK5 than with CK5/6 (P = .0001).

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Details

Title: CK5 is more sensitive than CK5/6 in identifying the "basal-like" phenotype of breast carcinoma
Creators: Rohit Bhargava - Magee-Womens Hospital
Sushil Beriwal
Kim McManus
David J Dabbs
Publication Details: American journal of clinical pathology, v 130(5), pp 724-730
Resource Type: Journal article
Language: English
Academic Unit: Radiation Oncology (and Nuclear Medicine)
Web of Science ID: WOS:000260188600007
Scopus ID: 2-s2.0-55249099328
Other Identifier: 991021897389204721

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Web of Science research areas: Pathology

CK5 is more sensitive than CK5/6 in identifying the "basal-like" phenotype of breast carcinoma

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