Journal article
CYTOKINE mRNA EXPRESSION IN HUMAN PLATELETS AND A MEGAKARYOCYTIC CELL LINE AND CYTOKINE MODULATION OF PLATELET FUNCTION
Cytokine (Philadelphia, Pa.), v 9(6), pp 405-411
1997
PMID: 9199874
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Abstract
Platelet formation and function are regulated, in vivo, to varying degrees by cytokines in the micro-environment. While white blood cells are the major source of cytokines within the cardiovascular system, the question addressed in this study was whether platelets and the platelet precursor, the megakaryocyte, may also serve as a source of cytokines. Cytokines produced by or carried within platelets could be released at sites of vascular injury and participate in wound healing. Platelets and a human megakaryocyte-like cell line, HU3, were found to express message for interleukin 7 (IL-7), stem cell factor (SCF), transforming growth factor beta (TGF-β), cMpl, the IgE receptor subunits FcϵRIαγ and the transcription factor, NF-E2. Other cytokines expressed in HU3 cells but not in platelets included IL-1β, IL-6, IL-10, IL-13, TNF-α and the FcϵRIβ subunit. The HU3 cell line seemed to be further along the maturation/differentiation pathway to platelet formation than a second blood derived bipotential cell line, MB02. The MB02 cell line did not express IL-6, IL-10, SCF, TNF-α nor cMpl. Furthermore, culturing the HU3 cells in TPO appeared to repress expression of FcϵRIβ directing the cell closer to the platelet phenotype. In light of the presence of cytokine expression in platelets/megakaryocytes, agonist-induced platelet aggregation was measured in the presence of added cytokines as a means to evaluate potential cytokine modulation of platelet function. Collagen-induced aggregations were significantly enhanced by IL-6, SCF and TPO. Other cytokines tested significantly stimulated the thrombin receptor activating peptide, SFLLRNP-, U46619- and ADP-induced platelet aggregations with TPO being the most consistent activator. It is possible that cytokines released from platelets act in concert with cytokines released from other cellular sources to modulate haemostasis and thrombosis differentially depending upon the site of injury.
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Details
- Title
- CYTOKINE mRNA EXPRESSION IN HUMAN PLATELETS AND A MEGAKARYOCYTIC CELL LINE AND CYTOKINE MODULATION OF PLATELET FUNCTION
- Creators
- Gerald SoslauDoris A MorganJonathan S JaffeIsadore BrodskyYihe Wang
- Publication Details
- Cytokine (Philadelphia, Pa.), v 9(6), pp 405-411
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- [Retired Faculty]
- Web of Science ID
- WOS:A1997XE21700004
- Scopus ID
- 2-s2.0-0031172191
- Other Identifier
- 991014878453404721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemistry & Molecular Biology
- Cell Biology
- Immunology