Journal article
Calcium phosphate-based biomaterials trigger human macrophages to release extracellular traps
Biomaterials, v 285, 121521
Jun 2022
PMID: 35523018
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
As central part of the innate immune response, immune cells fight against invaders through various mechanisms, such as the release of extracellular traps (ETs). While this mechanism is mainly known for neutrophils in biomaterial contact, the release of macrophage extracellular traps (METs) in response to biomaterials has not yet been reported. An important application area for biomaterials is bone, where healing of defects of a critical size requires the implantation of grafts, which are often composed of calcium phosphates (CaPs). In this study, the response of human monocyte-derived macrophages in vitro to two different CaPs (α-tricalcium phosphate (α-TCP) and calcium deficient hydroxyapatite (CDHA)) as well as different pore structures was investigated. Scaffolds with anisotropic porosity were prepared by directional freezing, while samples with isotropic pore structure served as reference. It was revealed that ETs are released by human monocyte-derived macrophages in direct or indirect contact with CaP scaffolds. This was caused by mineral nanoparticles formed during incubation of α-TCP samples in culture medium supplemented with human platelet lysate, with an anisotropic pore structure attenuating MET formation. METs were significantly less pronounced or absent in association with CDHA samples. It was furthermore demonstrated that MET formation was accompanied by an increase in pro-inflammatory cytokines. Thus, this study provided the first evidence that macrophages are capable of releasing ETs in response to biomaterials.
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Details
- Title
- Calcium phosphate-based biomaterials trigger human macrophages to release extracellular traps
- Creators
- Annika Seifert - University of WürzburgTina Tylek - University of WürzburgCarina Blum - University of WürzburgNaomi Hemmelmann - University of WürzburgBettina Böttcher - University of WürzburgUwe Gbureck - University of WürzburgJürgen Groll - University of Würzburg
- Publication Details
- Biomaterials, v 285, 121521
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000799379700002
- Scopus ID
- 2-s2.0-85129276924
- Other Identifier
- 991019292127704721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Engineering, Biomedical
- Materials Science, Biomaterials