Journal article
Calcium signaling of in situ chondrocytes in articular cartilage under compressive loading: Roles of calcium sources and cell membrane ion channels
Journal of orthopaedic research, v 36(2), pp 730-738
Feb 2018
PMID: 28980722
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Mechanical loading on articular cartilage can induce many physical and chemical stimuli on chondrocytes residing in the extracellular matrix (ECM). Intracellular calcium ([Ca
]
) signaling is among the earliest responses of chondrocytes to physical stimuli, but the [Ca
]
signaling of in situ chondrocytes in loaded cartilage is not fully understood due to the technical challenges in [Ca
]
imaging of chondrocytes in a deforming ECM. This study developed a novel bi-directional microscopy loading device that enables the record of transient [Ca
]
responses of in situ chondrocytes in loaded cartilage. It was found that compressive loading significantly promoted [Ca
]
signaling in chondrocytes with faster [Ca
]
oscillations in comparison to the non-loaded cartilage. Seven [Ca
]
signaling pathways were further investigated by treating the cartilage with antagonists prior to and/or during the loading. Removal of extracellular Ca
ions completely abolished the [Ca
]
responses of in situ chondrocytes, suggesting the indispensable role of extracellular Ca
sources in initiating the [Ca
]
signaling in chondrocytes. Depletion of intracellular Ca
stores, inhibition of PLC-IP
pathway, and block of purinergic receptors on plasma membrane led to significant reduction in the responsive rate of cells. Three types of ion channels that are regulated by different physical signals, TRPV4 (osmotic and mechanical stress), T-type VGCCs (electrical potential), and mechanical sensitive ion channels (mechanical loading) all demonstrated critical roles in controlling the [Ca
]
responses of in situ chondrocyte in the loaded cartilage. This study provided new knowledge about the [Ca
]
signaling and mechanobiology of chondrocytes in its natural residing environment. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:730-738, 2018.
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Details
- Title
- Calcium signaling of in situ chondrocytes in articular cartilage under compressive loading: Roles of calcium sources and cell membrane ion channels
- Creators
- Mengxi Lv - Department of Mechanical EngineeringUniversity of Delaware130 Academy StreetNewark19716DelawareYilu Zhou - Department of Mechanical EngineeringUniversity of Delaware130 Academy StreetNewark19716DelawareXingyu Chen - Department of Mechanical EngineeringUniversity of Delaware130 Academy StreetNewark19716DelawareLin Han - Drexel UniversityLiyun Wang - Department of Mechanical EngineeringUniversity of Delaware130 Academy StreetNewark19716DelawareX Lucas Lu - Department of Mechanical EngineeringUniversity of Delaware130 Academy StreetNewark19716Delaware
- Publication Details
- Journal of orthopaedic research, v 36(2), pp 730-738
- Publisher
- Wiley
- Grant note
- R01 AR054385 / NIAMS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000426733700021
- Scopus ID
- 2-s2.0-85032920004
- Other Identifier
- 991019168385604721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Orthopedics