Journal article
Carbon Monoxide Releasing Molecule-2 Enhances Coagulation and Diminishes Fibrinolytic Vulnerability in Plasma Exposed to Heparin or Argatroban
Anesthesia and analgesia, v 111(6), pp 1347-1352
01 Dec 2010
PMID: 21048101
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
BACKGROUND: It has been recently demonstrated that a carbon monoxide releasing molecule (tricarbonyldichlororuthenium [II] dimer; CORM-2) enhances coagulation and attenuates vulnerability to fibrinolysis in normal and hemophiliac human plasma. We tested the hypothesis that plasma anticoagulated with heparin or argatroban would demonstrate improved coagulation and decreased fibrinolytic vulnerability after exposure to CORM-2.
METHODS: Normal plasma was anticoagulated with 0 to 0.1 mu g/mL unfractionated heparin or 0 to 1 mu g/mL argatroban. Samples were subsequently exposed to 0 or 100 mu M CORM-2 and activated with tissue factor. Additional samples with the same anticoagulant and CORM-2 exposure schema were incubated with 100 U/mL tissue-type plasminogen activator (tPA) to assess fibrinolytic vulnerability. Thrombelastographic data were collected until either clot strength stabilized or clot lysis occurred as appropriate.
RESULTS: In the absence of tPA, CORM-2 significantly increased the velocity of clot growth in heparin (75%) and argatroban-exposed (40%) samples. Clot strength was also significantly increased in heparin (69%) and argatroban-exposed (72%) samples. In the presence of tPA, CORM-2-treated samples had even greater (94%-731%) increases in velocity of growth and strength after exposure to either anticoagulant and significantly increased clot lysis time (103%-200%).
CONCLUSIONS: CORM-2 exposure resulted in faster-growing, stronger, longer-lived thrombi after anticoagulation with heparin or argatroban. Additional preclinical investigation is warranted to determine whether CORM-2 administration will be useful in attenuating bleeding complications associated with thromboprophylaxis.
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Details
- Title
- Carbon Monoxide Releasing Molecule-2 Enhances Coagulation and Diminishes Fibrinolytic Vulnerability in Plasma Exposed to Heparin or Argatroban
- Creators
- Vance G. Nielsen - Drexel UniversityEjaz S. Khan - Drexel UniversityJames K. Kirklin - University of Alabama at BirminghamJames F. George - University of Alabama at Birmingham
- Publication Details
- Anesthesia and analgesia, v 111(6), pp 1347-1352
- Publisher
- Lippincott Williams & Wilkins
- Number of pages
- 6
- Resource Type
- Journal article
- Language
- English
- Web of Science ID
- WOS:000284973300003
- Scopus ID
- 2-s2.0-78650304606
- Other Identifier
- 991019357763304721
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InCites Highlights
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Anesthesiology