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Carbon Nanotube‐Tipped Endoscope for In Situ Intracellular Surface‐Enhanced Raman Spectroscopy
Journal article   Peer reviewed

Carbon Nanotube‐Tipped Endoscope for In Situ Intracellular Surface‐Enhanced Raman Spectroscopy

Jun Jie Niu, Michael G Schrlau, Gary Friedman and Yury Gogotsi
Small (Weinheim an der Bergstrasse, Germany), v 7(4), pp 540-545
18 Feb 2011
PMID: 21360811

Abstract

carbon nanotube Raman spectroscopy cell SERS endoscope
Gold nanoparticle‐decorated carbon nanotubes (CNTs) are used to study intracellular environments in situ using surface‐enhanced Raman spectroscopy (SERS). CNTs are decorated with gold nanoparticles and assembled onto the tips of pulled glass capillaries to form a SERS‐enabled endoscope. The sub‐micrometer size and high mechanical strength of the endoscope make it possible to penetrate the cell membrane for intracellular probing and remain positioned inside during lengthy SERS measurements without causing damage to the cell. Using the SERS‐enabled endoscope, DNA and other biomolecules are detected in situ within the nucleus of a single human cervical carcinoma cell in a minimally invasive manner. The SERS‐enabled endoscopes exhibit high selectivity and sensitivity for detecting trace amounts of analytes (≈1 pM) in biofluid environments, highlighting their capabilities as label‐free, biological sensors for real‐time in situ cellular diagnostics, biological detection, and pharmaceutical research. Carbon nanotubes decorated with gold nanoparticles form a surface‐enhanced Raman spectroscopy (SERS)‐enabled endoscope. The SERS‐enabled endoscope is used to detect DNA and other biomolecules in situ within the nucleus of a single human cervical carcinoma cell in a minimally invasive manner.

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Web of Science research areas
Chemistry, Multidisciplinary
Chemistry, Physical
Materials Science, Multidisciplinary
Nanoscience & Nanotechnology
Physics, Applied
Physics, Condensed Matter
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