Journal article
Carbon monoxide-releasing molecule-2 decreases fibrinolysis in human plasma
Blood coagulation & fibrinolysis, v 20(6), pp 448-455
Sep 2009
PMID: 19581800
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Carbon monoxide, derived from carbon monoxide-releasing molecules, has been recently demonstrated to enhance the velocity of formation and strength of plasma thrombi. We tested the hypothesis that carbon monoxide-releasing molecule-2 would modulate fibrinolysis of plasma thrombi. Normal plasma was exposed to 0, 25, 50, 100 or 200 micromol/l carbon monoxide-releasing molecule-2, with coagulation activated with tissue factor and fibrinolysis initiated with tissue-type plasminogen activator. Additional experiments utilized factor XIII, plasminogen activator inhibitor-1, thrombin activatable fibrinolysis inhibitor or alpha2-antiplasmin-deficient plasmas. Thrombus growth/disintegration kinetics was monitored with thrombelastography. Carbon monoxide-releasing molecule-2, in a concentration-dependent fashion, increased the velocity of thrombus formation and strength, and markedly attenuated fibrinolysis in normal plasma. In factor XIII-deficient plasma, carbon monoxide-releasing molecule-2 mediated effects on thrombus growth/disintegration kinetics were similar to that seen with normal plasma; however, carbon monoxide-releasing molecule-2 had a less marked effect on thrombus growth/disintegration in both plasminogen activator inhibitor-1 and thrombin activatable fibrinolysis inhibitor-deficient plasma, with even less carbon monoxide-releasing molecule-2-mediated effects noted in alpha2-antiplasmin-deficient plasma. Carbon monoxide-releasing molecule-2 attenuated fibrinolysis by enhancing the velocity of clot growth and strength while augmenting the effects of plasminogen activator inhibitor-1, thrombin activatable fibrinolysis inhibitor and alpha2-antiplasmin. These findings serve as the rationale for further investigations to determine if carbon monoxide-releasing molecules could be utilized as hemostatic agents.
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Details
- Title
- Carbon monoxide-releasing molecule-2 decreases fibrinolysis in human plasma
- Creators
- Vance G Nielsen - University of Alabama at BirminghamJames K Kirklin - University of Alabama at BirminghamJames F George - University of Alabama at Birmingham
- Publication Details
- Blood coagulation & fibrinolysis, v 20(6), pp 448-455
- Publisher
- Lippincott
- Resource Type
- Journal article
- Language
- English
- Web of Science ID
- WOS:000269330200011
- Scopus ID
- 2-s2.0-69549108991
- Other Identifier
- 991019357632904721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Hematology