Journal article
Carbon monoxide releasing molecule-2 enhances alpha(2)-antiplasmin activity
Blood coagulation & fibrinolysis, v 22(4), pp 345-348
01 Jun 2011
PMID: 21330914
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The objective of this study was to determine whether carbon monoxide releasing molecule (tricarbonyldichlororuthenium (II) dimer, CORM-2) directly affects alpha(2)-antiplasmin activity. For this purpose, purified alpha(2)-antiplasmin was exposed to 0 or 100 mu mol/l CORM-2 for 5 min at 37 degrees C and then placed in alpha(2)-antiplasmin-deficient plasma (25 mu g/ml alpha(2)-antiplasmin and 3.3 mu mol/l CORM-2 final concentrations). In a second series of experiments, alpha(2)-antiplasmin and deficient plasma were combined and then exposed to 0 or 100 mu mol/l CORM-2. Coagulation was activated with tissue factor and fibrinolysis initiated with tissue-type plasminogen activator (n = 8 per condition). Thrombus growth/disintegration kinetics were monitored with thrombelastography until clot lysis time occurred. Samples containing alpha(2)-antiplasmin preexposed to 100 mu mol/l CORM-2 demonstrated no changes in the velocity of clot growth, but had a significant prolongation of the time to maximum rate of lysis, clot lysis time, and a significant decrease in the maximum rate of clot lysis compared with samples preexposed to 0 mu mol/l CORM-2. In sharp contrast, addition of 100 mu mol/l CORM-2 to premixed alpha(2)-antiplasmin in its deficient plasma resulted in significant, marked increases in the velocity of clot growth and the strength with concurrent antifibrinolytic effects as in the first series. In conclusion, CORM-2 exerts its antifibrinolytic effects by direct enhancement of alpha(2)-antiplasmin activity. It appears that combined modification of both fibrinogen and alpha(2)-antiplasmin are responsible for the robust procoagulant/antifibrinolytic effects of CORM-2 in the fibrinolytic environment. Blood Coagul Fibrinolysis 22:345-348 (C) 2011 Wolters Kluwer Health | Lippincott Williams & Wilkins.
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Details
- Title
- Carbon monoxide releasing molecule-2 enhances alpha(2)-antiplasmin activity
- Creators
- Saninuj N. Malayaman - Hahnemann University HospitalJack B. Cohen - Drexel UniversityKelly A. Machovec - Drexel UniversityBrian E. Bernhardt - Drexel UniversityMatt R. Arkebauer - Drexel UniversityVance G. Nielsen - Drexel University
- Publication Details
- Blood coagulation & fibrinolysis, v 22(4), pp 345-348
- Publisher
- Lippincott Williams & Wilkins
- Number of pages
- 4
- Grant note
- Department of Anesthesiology Drexel University College of Medicine
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Anesthesiology and Perioperative Medicine
- Web of Science ID
- WOS:000290303300018
- Scopus ID
- 2-s2.0-79955945553
- Other Identifier
- 991019168012204721
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- Web of Science research areas
- Hematology