Journal article
Carbon monoxide-releasing molecule-2 enhances coagulation in rabbit plasma and decreases bleeding time in clopidogrel/aspirin-treated rabbits
Blood coagulation & fibrinolysis, v 22(8), pp 756-759
01 Dec 2011
PMID: 22008906
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Administration of carbon monoxide derived from carbon monoxide-releasing molecules has been demonstrated to enhance coagulation in vitro at small concentrations (100-200 mu mol/l) in human and rabbit plasma. We sought to determine if carbon monoxide-releasing molecule-2 [tricarbonyldichlororuthenium (II) dimer, CORM-2] would improve coagulation in rabbit plasma in vitro via thrombelastography and in an in vivo preclinical rabbit model of ear bleeding time following administration of clopidogrel (20 mg/kg) with aspirin (10 mg/kg) via gavage. Addition of 100 mu mol/l CORM-2 to rabbit plasma significantly improved coagulation. This procoagulant effect was blocked by pre-exposure of plasma to an agent that converts hemefibrinogen to methemefibrinogen in human plasma, preventing carbon monoxide binding and enhancement of coagulation. Rabbit ear bleeding time was 5.8 +/- 1.1 min 2-3 h after clopidogrel/aspirin administration. Bleeding time significantly decreased to 2.6 +/- 0.6 min, 5 min after administration of CORM-2 (10 mg/kg; 279 mu mol/l 'best-case' instantaneous concentration) intravenously. CORM-2 enhances plasmatic coagulation in a manner similar to that of human plasma in vitro, and plasmatic coagulation is enhanced in vivo by CORM-2 as well. Additional preclinical investigation of the effects of CORM-2 on coagulopathy (e.g. heparin or hemodilution mediated) utilizing this rabbit model is planned. Blood Coagul Fibrinolysis 22:756-759 (C) 2011 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.
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Details
- Title
- Carbon monoxide-releasing molecule-2 enhances coagulation in rabbit plasma and decreases bleeding time in clopidogrel/aspirin-treated rabbits
- Creators
- Vance G. Nielsen - Drexel UniversityNikhil Chawla - Drexel UniversityDipty Mangla - Drexel UniversitySheldon B. Gomes - Drexel UniversityMatthew R. Arkebauer - Drexel UniversityKimberly A. Wasko - Drexel UniversityKesavan Sadacharam - Drexel UniversityKeith Vosseller - Drexel University
- Publication Details
- Blood coagulation & fibrinolysis, v 22(8), pp 756-759
- Publisher
- Lippincott Williams & Wilkins
- Number of pages
- 4
- Grant note
- 4100054846 / Pennsylvania Department of Health Department of Anesthesiology
- Resource Type
- Journal article
- Language
- English
- Web of Science ID
- WOS:000297138400023
- Scopus ID
- 2-s2.0-84855199766
- Other Identifier
- 991019357774904721
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- Web of Science research areas
- Hematology