Journal article
Carbon monoxide releasing molecule-2 increases fibrinogen-dependent coagulation kinetics but does not enhance prothrombin activity
Blood coagulation & fibrinolysis, v 21(4), pp 349-353
01 Jun 2010
PMID: 20449889
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
We have previously determined that tricarbonyldichlororuthenium (II) dimer (CORM-2) increases plasma clot velocity of formation and strength by enhancing thrombin-fibrinogen interactions as determined by thrombelastography. The purpose of the present investigation was to further define the nature of CORM-2 interaction with prothrombin and fibrinogen by exposing purified proteins to CORM-2 or generating protein concentration-response curves in the absence or presence of CORM-2. Purified prothrombin was exposed to 0 or 100 mu mol/l CORM-2 prior to being added to prothrombin-deficient plasma (n=7-8 per condition). Fibrinogen-deficient plasma had fibrinogen added for a final concentration of 100-800 mg/dl and was exposed to 0 or 100 mu mol/l CORM-2 (n=4 per condition). Following tissue factor activation, thrombelastographic data were collected until clot strength stabilized. Exposure of prothrombin to CORM-2 did not significantly enhance coagulation kinetics. In sharp contrast, CORM-2 exposure enhanced fibrinogen coagulation kinetics in a concentration-dependent fashion, with peak effect seen at a fibrinogen concentration of 300 mg/dl that then progressively decreased throughout the range tested. Our data demonstrate that CORM-2 does not enhance plasma coagulation kinetics by modifying prothrombin; instead, the concept that CORM-2 modifies fibrinogen is the most likely explanation for the enhanced thrombin-fibrinogen interactions observed. Blood Coagul Fibrinolysis 21:349-353 (C) 2010 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.
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Details
- Title
- Carbon monoxide releasing molecule-2 increases fibrinogen-dependent coagulation kinetics but does not enhance prothrombin activity
- Creators
- Vance G. Nielsen - University of Alabama at BirminghamS. Nini Malayaman - University of Alabama at BirminghamEjaz S. Khan - University of Alabama at BirminghamJames K. Kirklin - Drexel UniversityJames F. George - Drexel University
- Publication Details
- Blood coagulation & fibrinolysis, v 21(4), pp 349-353
- Publisher
- Lippincott Williams & Wilkins
- Number of pages
- 5
- Grant note
- Departments of Anesthesiology and Surgery
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Anesthesiology and Perioperative Medicine
- Web of Science ID
- WOS:000277925800009
- Scopus ID
- 2-s2.0-77951902971
- Other Identifier
- 991019357637304721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Hematology