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Carbon nanopipettes characterize calcium release pathways in breast cancer cells
Journal article   Open access   Peer reviewed

Carbon nanopipettes characterize calcium release pathways in breast cancer cells

Michael G. Schrlau, Eugen Brailoiu, Sandip Patel, Yury Gogotsi, Nae J. Dun and Haim H. Bau
Nanotechnology, v 19(32), 325102
13 Aug 2008
DOI: https://doi.org/10.1088/0957-4484/19/32/325102
PMID: 21828806
url
https://repository.upenn.edu/meam_papers/154View
Submitted Open Open Access (License Unspecified)

Abstract

Materials Science, Multidisciplinary Nanoscience & Nanotechnology Physics, Applied Science & Technology Science & Technology - Other Topics Materials Science Physical Sciences Physics Technology
Carbon-based nanoprobes are attractive for minimally invasive cell interrogation but their application in cell physiology has thus far been limited. We have developed carbon nanopipettes (CNPs) with nanoscopic tips and used them to inject calcium-mobilizing messengers into cells without compromising cell viability. We identify pathways sensitive to cyclic adenosine diphosphate ribose (cADPr) and nicotinic acid adenine dinucleotide phosphate ( NAADP) in breast carcinoma cells. Our findings demonstrate the superior utility of CNPs for intracellular delivery of impermeant molecules and, more generally, for cell physiology studies. The CNPs do not appear to cause any lasting damage to cells. Their advantages over commonly used glass pipettes include smaller size, breakage and clogging resistance, and potential for multifunctionality such as in concurrent injection and electrical measurements.

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34 citations in Web of Science
38 citations in Scopus

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Collaboration types
Domestic collaboration
International collaboration
Web of Science research areas
Materials Science, Multidisciplinary
Nanoscience & Nanotechnology
Physics, Applied
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