Journal article
Caveolin-1 in situ expression in glomerular and peritubular capillaries as a marker of ultrastructural progression and severity of renal thrombotic microangiopathy
Journal of nephrology, v 36(8), pp 2327-2333
01 Nov 2023
PMID: 37480399
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Background Thrombotic microangiopathy is a severe and potentially life-threatening condition inducing severe endothelial injury in many organs, particularly native and transplanted kidneys. Current pathological studies by our group have identified the use of Caveolin-1 immunohistochemistry as a potential marker of endothelial damage and progression degree of thrombotic microangiopathy. The aim of the present work was to evaluate Caveolin-1 as a marker of severity in thrombotic microangiopathy kidney disease, according to the ultrastructural progression of the disease evaluated by transmission electron microscopy.
Materials and methods Twenty-nine patients (17 non-transplanted and 12 transplanted) were retrospectively selected, biopsied for suspected or histologically-confirmed thrombotic microangiopathy. Transmission electron microscopy was performed in all cases, and an ultrastructural score of thrombotic microangiopathy-related glomerular disease was assessed (from 0 to 3+). Immunohistochemistry for Caveolin-1 was automatically performed.
Results The mean percentage of Caveolin-1-positive glomerular capillaries was 53.2 +/- 40.6% and 28.0 +/- 42.8% in the active thrombotic microangiopathy versus previous thrombotic microangiopathy cases (p = 0.085), considering both native and transplanted kidneys. The presence of progressive disease correlated with diffuse Caveolin-1 immunoreactivity (p = 0.031), and ultrastructural score correlated with glomerular Caveolin-1 positivity, progressively increasing from 22.5% of the Score 0 group to 95.5% of the Score 3 group (p = 0.036).
Discussion Caveolin-1 proved to be a very useful marker of early endothelial damage in the course of thrombotic microangiopathy for both native and transplanted kidneys, therefore worth considering in routine practice. Diffuse glomerular Caveolin-1 immunoreactivity correlates with the severity of the thrombotic disease and it can appear very early, even before ultrastructurally evident endothelial damage.
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Details
- Title
- Caveolin-1 in situ expression in glomerular and peritubular capillaries as a marker of ultrastructural progression and severity of renal thrombotic microangiopathy
- Creators
- Francesco Vasuri - Azienda USL di BolognaAnthony P. Lisi - Drexel UniversityCarmen Ciavarella - University of BolognaAlessio Degiovanni - Azienda USL di BolognaBenedetta Fabbrizio - Azienda USL di BolognaSabrina Valente - University of BolognaGisella Vischini - University of BolognaGaetano La Manna - University of BolognaAntonia D'Errico - University of BolognaGianandrea Pasquinelli - University of Bologna
- Publication Details
- Journal of nephrology, v 36(8), pp 2327-2333
- Publisher
- Springer Nature
- Number of pages
- 7
- Grant note
- RC-2022-2773443 / Italian Ministry of Health; Ministry of Health, Italy
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemistry; Drexel University
- Web of Science ID
- WOS:001035746500001
- Scopus ID
- 2-s2.0-85165442120
- Other Identifier
- 991021861281204721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Urology & Nephrology