Journal article
Cell-Free Identification of S-cerevisiae Strains by Analysis of Supernatant Using LC-MS
Journal of the American Society for Mass Spectrometry, v 29(11), pp 2260-2267
01 Nov 2018
PMID: 30105741
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Current literature shows a gap for methods which can identify yeast sub-species (strains or serovars) in samples where there are no viable cells remaining. Presented here is a technique for the analysis of yeast supernatant, including solid phase extraction, data-dependent acquisition liquid chromatography/mass spectrometry (LC-MS), and two chemometric methods to identify and classify yeast strains. Five strains of Saccharomyces cerevisiae were successfully identified in various stages of growth. In addition, peptide/protein identification was performed, without the need for additional data acquisition.
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Details
- Title
- Cell-Free Identification of S-cerevisiae Strains by Analysis of Supernatant Using LC-MS
- Creators
- Cathy Muste - Drexel UniversityKevin G. Owens - Drexel University
- Publication Details
- Journal of the American Society for Mass Spectrometry, v 29(11), pp 2260-2267
- Publisher
- Springer Nature
- Number of pages
- 8
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemistry
- Web of Science ID
- WOS:000447520900015
- Scopus ID
- 2-s2.0-85054893239
- Other Identifier
- 991019169625604721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemical Research Methods
- Chemistry, Analytical
- Chemistry, Physical
- Spectroscopy