Journal article
Cell-cell conjugation. Transient analysis and experimental implications
Biophysical journal, v 58(3), pp 641-652
Sep 1990
PMID: 2207256
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
In the present study we investigate the transient conjugation of cell pairs by using a mathematical model. Macromolecules responsible for adhesion (bonds) are assumed to exist in two reversible states, attached and unattached, and exert a force elastic in nature only when they cross-link the two cell surfaces (attached state). Bonds form a link between the two cell surfaces only in the attached form. The unattached bridges are assumed laterally mobile in the plane of the cell membrane. Lateral mobility of attached bonds may be limited by structures on the undersurface of the cell membrane. Using this model we show that the bond density distribution between a cytotoxic T-cell (F-1) and a cancer cell (JY:HLA-A2-B7-DR4, W6) approaches equilibrium within 10 min, the incubation period used in experiments by Sung, K.L.P., L.A. Sung, M. Crimmins, S.J. Burakoff, and S. Chien (1986. Science [Wash. DC]. 234:1405-1408). If the diffusion coefficient of attached bonds is set equal to zero in the computations the model predictions indicate accumulation of bonds at the edge of conjugation. This prediction is consistent with present experimental data on lectin-induced red blood cell aggregation (Vayo, M., R. Skalak, P. Brunn, S. Usami, and S. Chien. 1987. Fed. Proc. 46:1043). It is concluded that significant features of micromanipulation data on specific adhesion can be explained by the diffusivity properties of bonds responsible for adhesion.
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Details
- Title
- Cell-cell conjugation. Transient analysis and experimental implications
- Creators
- A Tozeren - Department of Mechanical Engineering, Catholic University of America, Washington, DC 20064
- Publication Details
- Biophysical journal, v 58(3), pp 641-652
- Publisher
- Elsevier; United States
- Grant note
- R01-GM41460 / NIGMS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- [Retired Faculty]
- Web of Science ID
- WOS:A1990DX50000007
- Scopus ID
- 2-s2.0-0025026017
- Other Identifier
- 991014878403704721
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- Web of Science research areas
- Biophysics