Journal article
Cell-level 3D reconstruction and quantification of the Drosophila wing imaginal disc
International journal of bioinformatics research and applications, v 15(2), pp 174-189
2019
Abstract
We describe a set of techniques that, when applied to a 3D stack of confocal microscopy images, produces a volumetric model of an epithelial tissue as well as a mesh model of its apicolateral cell boundaries. Via a projection step, detailed 3D models that approximate the individual cells in the epithelium are then defined. Once the individual cells are generated, their apical face area, height and volume may be computed and visualised, providing quantitative and visual data about the patterns of cells within the tissue. We have applied the techniques to the analysis of the developing wing imaginal disc of a late-larval Drosophila melanogaster. Our techniques are being applied to a series of specimens in an investigation that intends to quantitatively substantiate observed cell shape changes that occur during wing imaginal disc development.
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2 citations in Scopus
Details
- Title
- Cell-level 3D reconstruction and quantification of the Drosophila wing imaginal disc
- Creators
- David E Breen - 1Department of Computer Science, Drexel University, Philadelphia, PA, USALiyuan Sui - 2Department of Biology, Technische Universität Dresden, Dresden, GermanyLinge Bai - 3Department of Computer Science, Drexel University, Philadelphia, PA, USAFrank Jülicher - 4Department of Biological Physics, Max Planck Institute for the Physics of Complex Systems, Dresden, GermanyChristian Dahmann - 5Department of Biology, Technische Universität Dresden, Dresden, Germany
- Publication Details
- International journal of bioinformatics research and applications, v 15(2), pp 174-189
- Publisher
- Inderscience Publishers (IEL)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Computer Science
- Scopus ID
- 2-s2.0-85065708789
- Other Identifier
- 991014878336604721