Journal article
Cell viability measurement using 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester and a cantilever sensor
Analytical chemistry (Washington), v 83(4), pp 1480-1483
15 Feb 2011
PMID: 21244099
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Detection of viable pathogenic bacteria has widespread application in food safety and human health. Antibody-based methods require a growth step which limits time-to-results performance. In this study, we use a mass-change sensitive cantilever biosensor and a probe, 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM), that accumulates only in live cells inducing a mass-change response to determine the cell viability in a short time. A poly-L-lysine coated sensor immobilized with live Escherichia coli JM101 (a surrogate for a pathogenic target) at various concentrations was exposed to BCECF-AM in a flow arrangement. A larger resonant frequency decrease in response to 100 μL of 60 μM BCECF-AM was observed when the sensor surface cell concentration was increased from 1 090 ± 580 to 3 960 ± 370 cells/mm(2) (n = 5). A log-linear relationship between the sensor surface cell concentration and frequency response was obtained in the range of 1 000-4 000 cells/mm(2) and as low as ∼2 000 viable E. coli cells were rapidly detected (<1 h).
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Details
- Title
- Cell viability measurement using 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester and a cantilever sensor
- Creators
- Sen Xu - Drexel UniversityRaj Mutharasan - Drexel University
- Publication Details
- Analytical chemistry (Washington), v 83(4), pp 1480-1483
- Publisher
- American Chemical Society; Washington, DC
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemical and Biological Engineering
- Web of Science ID
- WOS:000287176900048
- Scopus ID
- 2-s2.0-79951640927
- Other Identifier
- 991019170854204721
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- Web of Science research areas
- Chemistry, Analytical